Persisted/recurrent hyperparathyroidism associated with development of multi-drug resistance phenotype and proliferation of parathyroid transplants.

  • Authors:
    • Tomasz Grzela
    • Witold Chudzinski
    • Maciej Lazarczyk
    • Justyna Niderla
    • Piotr Dziunycz
    • Lukasz Milewski
    • Robert Samaha
    • Malgorzata Kobylecka
    • Joanna Miskiewicz
    • Elzbieta Bogacka-Zatorska
    • Barbara Gornicka
    • Pawel Swiercz
    • Magdalena Jelenska
    • Leszek Krolicki
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijmm.14.4.595
  • Pages: 595-604
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Abstract

The surgical treatment of secondary hyperparathyroidism (HPTH) requires sub-total excision of parathyroid glands or total excision with their autotransplantation. Although this approach has been considered as a safe method of treatment, in this report we describe persisted/recurrent HPTH after parathyroid transplantation. Due to parathormone (PTH) hypersecretion and uncontrolled proliferation, the parathyroid grafts were removed and used for generation of cell cultures, which further have been subjected to in vitro studies. As a control we used parathyroid tissue, obtained during multiorgan harvesting. We found increased proliferation and up-regulated PTH production by the graft-derived, but not control in vitro cultured cells. Moreover, due to decrease of in vivo radiotracer uptake by parathyroid grafts, the expression of multi-drug resistance-involved factors, including P-glycoprotein (P-gp/mdr1), multi-drug resistance-associated protein (mrp) and bcl-2 have been investigated using RT-PCR. The analysis revealed increased expression of both, mdr1 and mrp in graft-derived cells, in contrast to control cells, which did not express P-gp/mdr1 or mrp. However, we did not observe any difference in expression of bcl-2 between analyzed cells. The up-regulated expression of P-gp/mdr1 on graft-derived cells was further confirmed by immunofluorescence studies. The described case indicates potential risk associated with transplantation of parathyroid tissue. Our results confirm a role of MDR phenomenon in occurrence of false negative results in parathyroid tissue scintigraphy studies. Moreover, they indicate that standard histological examination of transplanted material could not be sensitive enough to exclude any potential danger of abnormal graft progression. Thus, they could support the concept to use encapsulated parathyroid transplants.

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October 2004
Volume 14 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Grzela T, Chudzinski W, Lazarczyk M, Niderla J, Dziunycz P, Milewski L, Samaha R, Kobylecka M, Miskiewicz J, Bogacka-Zatorska E, Bogacka-Zatorska E, et al: Persisted/recurrent hyperparathyroidism associated with development of multi-drug resistance phenotype and proliferation of parathyroid transplants.. Int J Mol Med 14: 595-604, 2004
APA
Grzela, T., Chudzinski, W., Lazarczyk, M., Niderla, J., Dziunycz, P., Milewski, L. ... Krolicki, L. (2004). Persisted/recurrent hyperparathyroidism associated with development of multi-drug resistance phenotype and proliferation of parathyroid transplants.. International Journal of Molecular Medicine, 14, 595-604. https://doi.org/10.3892/ijmm.14.4.595
MLA
Grzela, T., Chudzinski, W., Lazarczyk, M., Niderla, J., Dziunycz, P., Milewski, L., Samaha, R., Kobylecka, M., Miskiewicz, J., Bogacka-Zatorska, E., Gornicka, B., Swiercz, P., Jelenska, M., Krolicki, L."Persisted/recurrent hyperparathyroidism associated with development of multi-drug resistance phenotype and proliferation of parathyroid transplants.". International Journal of Molecular Medicine 14.4 (2004): 595-604.
Chicago
Grzela, T., Chudzinski, W., Lazarczyk, M., Niderla, J., Dziunycz, P., Milewski, L., Samaha, R., Kobylecka, M., Miskiewicz, J., Bogacka-Zatorska, E., Gornicka, B., Swiercz, P., Jelenska, M., Krolicki, L."Persisted/recurrent hyperparathyroidism associated with development of multi-drug resistance phenotype and proliferation of parathyroid transplants.". International Journal of Molecular Medicine 14, no. 4 (2004): 595-604. https://doi.org/10.3892/ijmm.14.4.595