Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101

  • Authors:
    • Masaki Hiramoto
    • Yutaka Kawakami
    • Ryusuke Nabeshima
    • Daisuke Shima
    • Hiroshi Handa
    • Shin Aizawa
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijmm.14.5.867
  • Pages: 867-872
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Abstract

We have previously reported that human promyelocytic leukemia HL-60 cells can be induced to differentiate into mature granulocytes when HL-60 co-cultivated with human bone marrow stromal LP101 cells. In the present study, we investigated which factors produced by LP101 cells induce HL-60 cells to differentiate into mature granulocytes. The expression of the cell surface antigen CD11b on HL-60 cells was increased after a 72-h culture with the conditioned medium (CM) obtained from LP101 cells. LP101 cells were observed to produce various cytokines, including TNF-α, GM-CSF and IL-6. The neutralizing antibodies against these cytokines partially suppressed the CM-induced differentiation of HL-60 cells. Recombinant TNF-α induced the differentiation of HL-60 cells, and GM-CSF and IL-6 additionally enhanced the effect of TNF-α. When the CM was divided into a low molecular weight (LMW) fraction and a high molecular weight (HMW) fraction by ultrafiltration, the LMW fraction synergistically enhanced the differentiation inducible activity of TNF-α. These results demonstrate that LP101 cells induce the differentiation of HL-60 cells by producing various cytokines including TNF-α, IL-6, and GM-CSF, and that unknown low molecular weight factors also participate.

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November 2004
Volume 14 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hiramoto M, Kawakami Y, Nabeshima R, Shima D, Handa H and Aizawa S: Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101. Int J Mol Med 14: 867-872, 2004.
APA
Hiramoto, M., Kawakami, Y., Nabeshima, R., Shima, D., Handa, H., & Aizawa, S. (2004). Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101. International Journal of Molecular Medicine, 14, 867-872. https://doi.org/10.3892/ijmm.14.5.867
MLA
Hiramoto, M., Kawakami, Y., Nabeshima, R., Shima, D., Handa, H., Aizawa, S."Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101". International Journal of Molecular Medicine 14.5 (2004): 867-872.
Chicago
Hiramoto, M., Kawakami, Y., Nabeshima, R., Shima, D., Handa, H., Aizawa, S."Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101". International Journal of Molecular Medicine 14, no. 5 (2004): 867-872. https://doi.org/10.3892/ijmm.14.5.867