Adrenomedullin and endothelin-1 stimulate in vitro expansion of cord blood hematopoietic stem cells

  • Authors:
    • Sergio De Angeli
    • Laura Del Pup
    • Erica Febas
    • Maria Teresa Conconi
    • Mara Tommasini
    • Rosa Di Liddo
    • Giovanna Albertin
    • Pier Paolo Parnigotto
    • Gastone G. Nussdorfer
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/ijmm.14.6.1083
  • Pages: 1083-1086
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Abstract

The improvement of techniques for in vitro expansion of cord blood (CB) hematopoietic stem cells (SCs) is, at present, one main task of tissue engineering. Hence, we investigated whether endothelin-1 (ET-1) and adrenomedullin (AM), two regulatory peptides exerting growth promoting action on several cell systems, favor the in vitro expansion of CB SCs in liquid culture. CB hematopoietic cell middle-term expansion was carried out in a stroma-free liquid culture medium in the presence of ET-1, AM and three different cytokine combinations. After two weeks of incubation, aliquots of expanded-cell suspension were seeded on semisolid medium and clonogenic tests were carried out by counting the number of colony forming units (CFUs) after 14 days of culture. Neither ET-1 nor AM (2.5x10−8 M) were per se able to significantly increase the CFU number, but both peptides magnified the pro-expansive effects of some cytokine cocktails. In light of these findings, we conclude that ET-1 and AM are to be considered novel promising molecules that, in association with cytokines, can be utilized as pro-expansive factors of CB SCs in prevision of their clinical use in allogeneic transplantation.

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December 2004
Volume 14 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
De Angeli S, Del Pup L, Febas E, Conconi MT, Tommasini M, Di Liddo R, Albertin G, Parnigotto PP and Nussdorfer GG: Adrenomedullin and endothelin-1 stimulate in vitro expansion of cord blood hematopoietic stem cells. Int J Mol Med 14: 1083-1086, 2004.
APA
De Angeli, S., Del Pup, L., Febas, E., Conconi, M.T., Tommasini, M., Di Liddo, R. ... Nussdorfer, G.G. (2004). Adrenomedullin and endothelin-1 stimulate in vitro expansion of cord blood hematopoietic stem cells. International Journal of Molecular Medicine, 14, 1083-1086. https://doi.org/10.3892/ijmm.14.6.1083
MLA
De Angeli, S., Del Pup, L., Febas, E., Conconi, M. T., Tommasini, M., Di Liddo, R., Albertin, G., Parnigotto, P. P., Nussdorfer, G. G."Adrenomedullin and endothelin-1 stimulate in vitro expansion of cord blood hematopoietic stem cells". International Journal of Molecular Medicine 14.6 (2004): 1083-1086.
Chicago
De Angeli, S., Del Pup, L., Febas, E., Conconi, M. T., Tommasini, M., Di Liddo, R., Albertin, G., Parnigotto, P. P., Nussdorfer, G. G."Adrenomedullin and endothelin-1 stimulate in vitro expansion of cord blood hematopoietic stem cells". International Journal of Molecular Medicine 14, no. 6 (2004): 1083-1086. https://doi.org/10.3892/ijmm.14.6.1083