Interleukin-1β and tumor necrosis factor-α upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts

  • Authors:
    • Zhuobin Zhang
    • Akira Andoh
    • Hirofumi Yasui
    • Osamu Inatomi
    • Kazunori Hata
    • Tomoyuki Tsujikawa
    • Katsuyuki Kitoh
    • Atsushi Takayanagi
    • Nobuyoshi Shimizu
    • Yoshihide Fujiyama
  • View Affiliations

  • Published online on: January 1, 2005     https://doi.org/10.3892/ijmm.15.1.79
  • Pages: 79-83
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Abstract

The recently identified cytokine interleukin-23 (IL-23) consists of p19 and p40 subunits. The major cellular source of IL-23 is dendritic cells and/or macrophages. We investigated the expression of IL-23 p19 mRNA in human colonic subepithelial myofibroblasts (SEMFs). p19 mRNA was not expressed in unstimulated SEMFs, but IL-1β and TNF-α strongly induced p19 mRNA expression in these cells. The effects of IL-1β were much stronger than those of TNF-α. These responses were observed in both a dose- and time-dependent manner. Furthermore, these cytokines acted synergistically when used in combination. A blockade of NF-κB activation by the overexpression of a stable form of IκBα completely blocked these responses, indicating that the induction of p19 mRNA expression by IL-1β and TNF-α was mediated by the NF-κB activation pathway. In conclusion, this is the first report demonstrating that IL-23 p19 mRNA is inducible in colonic myofibroblasts by IL-1β and TNF-α. The p19 expression in these cells might play a role in mucosal immune responses.

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January 2005
Volume 15 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang Z, Andoh A, Yasui H, Inatomi O, Hata K, Tsujikawa T, Kitoh K, Takayanagi A, Shimizu N, Fujiyama Y, Fujiyama Y, et al: Interleukin-1β and tumor necrosis factor-α upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts. Int J Mol Med 15: 79-83, 2005
APA
Zhang, Z., Andoh, A., Yasui, H., Inatomi, O., Hata, K., Tsujikawa, T. ... Fujiyama, Y. (2005). Interleukin-1β and tumor necrosis factor-α upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts. International Journal of Molecular Medicine, 15, 79-83. https://doi.org/10.3892/ijmm.15.1.79
MLA
Zhang, Z., Andoh, A., Yasui, H., Inatomi, O., Hata, K., Tsujikawa, T., Kitoh, K., Takayanagi, A., Shimizu, N., Fujiyama, Y."Interleukin-1β and tumor necrosis factor-α upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts". International Journal of Molecular Medicine 15.1 (2005): 79-83.
Chicago
Zhang, Z., Andoh, A., Yasui, H., Inatomi, O., Hata, K., Tsujikawa, T., Kitoh, K., Takayanagi, A., Shimizu, N., Fujiyama, Y."Interleukin-1β and tumor necrosis factor-α upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts". International Journal of Molecular Medicine 15, no. 1 (2005): 79-83. https://doi.org/10.3892/ijmm.15.1.79