Fish soluble Toll-like receptor 5 (TLR5S) is an acute-phase protein with integral flagellin-recognition activity

  • Authors:
    • Hironobu Tsukada
    • Aya Fukui
    • Tadayuki Tsujita
    • Misako Matsumoto
    • Takaji Iida
    • Tsukasa Seya
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijmm.15.3.519
  • Pages: 519-525
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Abstract

From an EST fragment of the rainbow trout that was predicted to contain leucine-rich repeats (LRR), we cloned the whole cDNA and identified a soluble form of TLR5 ortholog (rtTLR5S), which does not exist in the mouse and human. rtTLR5S was about 38% homologous to the extracellular domains of human (hu) and mouse (mo)TLR5, while rtTLR5S showed <25% homologous to those of other human or mouse TLRs. A chimera constructed of rtTLR5S and the intra-cellular TIR of huTLR5 expressed on HeLa cells signaled the presence of flagellin A and C from V. anguillarum, resulting in NF-κB activation. The mRNA of rtTLR5S was predominantly detected in the liver. The hepatoma cell line of the rainbow trout RTH149 that responded to flagellin, allowed to up-regulate rtTLR5S in response to V. anguillarum or its purified flagellin within 8 h. rtTLR5S, when co-expressed with membrane huTLR5 in HeLa cells, augmented huTLR5-mediated NF-κB activation in response to flagellin. These results, together with the genome information of the pufferfish Fugu (Fugu rubripes), suggest that in fish the soluble TLR5 is an acute-phase protein sensing bacterial infection via recognition of a variety of bacterial flagellins to augment NF-κB activation, and may be important for fish to survive from bacterial infection in the water.

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March 2005
Volume 15 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tsukada H, Fukui A, Tsujita T, Matsumoto M, Iida T and Seya T: Fish soluble Toll-like receptor 5 (TLR5S) is an acute-phase protein with integral flagellin-recognition activity. Int J Mol Med 15: 519-525, 2005
APA
Tsukada, H., Fukui, A., Tsujita, T., Matsumoto, M., Iida, T., & Seya, T. (2005). Fish soluble Toll-like receptor 5 (TLR5S) is an acute-phase protein with integral flagellin-recognition activity. International Journal of Molecular Medicine, 15, 519-525. https://doi.org/10.3892/ijmm.15.3.519
MLA
Tsukada, H., Fukui, A., Tsujita, T., Matsumoto, M., Iida, T., Seya, T."Fish soluble Toll-like receptor 5 (TLR5S) is an acute-phase protein with integral flagellin-recognition activity". International Journal of Molecular Medicine 15.3 (2005): 519-525.
Chicago
Tsukada, H., Fukui, A., Tsujita, T., Matsumoto, M., Iida, T., Seya, T."Fish soluble Toll-like receptor 5 (TLR5S) is an acute-phase protein with integral flagellin-recognition activity". International Journal of Molecular Medicine 15, no. 3 (2005): 519-525. https://doi.org/10.3892/ijmm.15.3.519