Polymorphisms and mutations of the folate receptor-alpha gene and risk of gastric cancer in a Chinese population

  • Authors:
    • Gui Zhang
    • Qing-Ying Zhang
    • Xiao-Ping Miao
    • Dong-Xin Lin
    • You-Yong Lu
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/ijmm.15.4.627
  • Pages: 627-632
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Abstract

Folate deficiency is associated with increased risk of many human diseases including several cancers. Folate receptor-alpha (FR-α) plays an important role in mediating influx of folates into cells and its expression can be induced by some environmental risk factors. Our previous study showed that decreased expression of FR-α occurs in human gastric cancer cell lines. In order to reveal the molecular mechanism of the difference of FR-α expression among these cell lines and analyze the relation between the mutations or/and polymorphisms of FR-α gene and gastric cancer, we screened the mutations of global FR-α gene in 12 gastric cancer cell lines and 28 gastric tumors and matched normal tissues by using PCR-DHPLC and DNA sequencing techniques, then further checked the exon2 of FR-α gene in 138 gastric tumors and matched normal tissues. Furthermore, we examined the relationship between two polymorphisms of FR-α A1314G and C1816delC and risk for gastric cancer in 296 cases and 354 age and sex-matched controls in northern China. We found that the allele frequency of FR-α1314A among cases was significantly higher than that among controls (0.186 vs. 0.143, P=0.014). Subjects with the A/G and A/A genotype had an increased risk for developing gastric cancer compared with the G/G genotype (OR=1.55; 95% CI=1.10-2.17). These results support the hypothesis that genetic polymorphism in the FR-α gene may contribute to susceptibility to carcinogenesis of the gastric cancer in the at-risk Chinese population.

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April 2005
Volume 15 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang G, Zhang Q, Miao X, Lin D and Lu Y: Polymorphisms and mutations of the folate receptor-alpha gene and risk of gastric cancer in a Chinese population. Int J Mol Med 15: 627-632, 2005
APA
Zhang, G., Zhang, Q., Miao, X., Lin, D., & Lu, Y. (2005). Polymorphisms and mutations of the folate receptor-alpha gene and risk of gastric cancer in a Chinese population. International Journal of Molecular Medicine, 15, 627-632. https://doi.org/10.3892/ijmm.15.4.627
MLA
Zhang, G., Zhang, Q., Miao, X., Lin, D., Lu, Y."Polymorphisms and mutations of the folate receptor-alpha gene and risk of gastric cancer in a Chinese population". International Journal of Molecular Medicine 15.4 (2005): 627-632.
Chicago
Zhang, G., Zhang, Q., Miao, X., Lin, D., Lu, Y."Polymorphisms and mutations of the folate receptor-alpha gene and risk of gastric cancer in a Chinese population". International Journal of Molecular Medicine 15, no. 4 (2005): 627-632. https://doi.org/10.3892/ijmm.15.4.627