Inhibition of proteasome activity in Gleditsia sinensis fruit extract-mediated apoptosis on human carcinoma cells

  • Authors:
    • Filly Cheung
    • Chung Hin Chui
    • Albert Sun Chi Chan
    • Fung Yi Lau
    • Gregory Yin Ming Cheng
    • Raymond Siu Ming Wong
    • Stanton Hon Lung Kok
    • Ivy Tuang Ngo Teo
    • Chor Hing Cheng
    • Johnny Cheuk On Tang
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/ijmm.16.5.925
  • Pages: 925-929
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Abstract

The anomalous fruit extract of Gleditsia sinensis (GSE) was shown to possess anticancer potential on various solid tumour and leukaemia cell lines in vitro. We have recently demonstrated that the mitochondrial-dependent apoptotic pathway including mitochondrial membrane potential depolarization, changes in the level of reactive oxygen species and activation of caspase 3 were recruited in GSE-induced apoptosis. Whether receptor-dependent APO-1/Fas apoptotic pathway is also involved remains uncertain. Using two solid tumour cell lines, the HepG2 hepatoblastoma carcinoma cells and MDA-MB231 breast cancer cells, we demonstrated that the Fas ligand and Fas receptor protein levels did not have significant variation after GSE incubation. Caspase 8 activity increased only weakly when compared with that of caspase 3. The chrymotrypsin-like activity of proteasome was partially inhibited up to 30-40% when compared with the untreated control. Taken together, we believe that GSE- mediated apoptosis on HepG2 and MDA-MB231 carcinoma cells is mainly dictated by the mitochondrial-dependent pathway while inhibition of proteasome activity may also be involved in GSE-induced apoptosis.

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November 2005
Volume 16 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Cheung F, Chui CH, Chan AS, Lau FY, Cheng GY, Wong RS, Kok SH, Teo IT, Cheng CH, Tang JC, Tang JC, et al: Inhibition of proteasome activity in Gleditsia sinensis fruit extract-mediated apoptosis on human carcinoma cells. Int J Mol Med 16: 925-929, 2005.
APA
Cheung, F., Chui, C.H., Chan, A.S., Lau, F.Y., Cheng, G.Y., Wong, R.S. ... Tang, J.C. (2005). Inhibition of proteasome activity in Gleditsia sinensis fruit extract-mediated apoptosis on human carcinoma cells. International Journal of Molecular Medicine, 16, 925-929. https://doi.org/10.3892/ijmm.16.5.925
MLA
Cheung, F., Chui, C. H., Chan, A. S., Lau, F. Y., Cheng, G. Y., Wong, R. S., Kok, S. H., Teo, I. T., Cheng, C. H., Tang, J. C."Inhibition of proteasome activity in Gleditsia sinensis fruit extract-mediated apoptosis on human carcinoma cells". International Journal of Molecular Medicine 16.5 (2005): 925-929.
Chicago
Cheung, F., Chui, C. H., Chan, A. S., Lau, F. Y., Cheng, G. Y., Wong, R. S., Kok, S. H., Teo, I. T., Cheng, C. H., Tang, J. C."Inhibition of proteasome activity in Gleditsia sinensis fruit extract-mediated apoptosis on human carcinoma cells". International Journal of Molecular Medicine 16, no. 5 (2005): 925-929. https://doi.org/10.3892/ijmm.16.5.925