Mutated-leptin gene transfer induces increases in body weight by electroporation and hydrodynamics-based gene delivery in mice

  • Authors:
    • Lan Xiang
    • Atsushi Murai
    • Tatsuo Muramatsu
  • View Affiliations

  • Published online on: December 1, 2005     https://doi.org/10.3892/ijmm.16.6.1015
  • Pages: 1015-1020
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Abstract

To investigate whether in vivo gene transfer causes leptin-antagonistic effects on food intake, animal body weight and fat tissue weight, the R128Q mutated-leptin gene, an R to Q substitution at position 128 of mouse leptin, was transferred into mouse liver and leg muscle by electroporation and hydrodynamics-based gene delivery. Mutated-leptin gene transfer by electroporation caused significant increases in body weight at 5 days and after (5.4% increase relative to control; p<0.05). Hydrodynamics-based gene delivery of the mutated-leptin gene also caused an increase in body weight (3.0% increase relative to control; p<0.05). Mutated-leptin gene transfer by electroporation significantly increased the tissue weight of epididymal white fat and neuropeptide Y mRNA expression in the hypothalamus compared with those of the control group 3 weeks after gene transfer (p<0.05). These results suggest that mutated-leptin gene transfer successfully produced leptin-antagonistic effects by modulating the central regulator of energy homeostasis. Also, the extent of leptin-antagonistic effects by electroporation was much higher than hydrodynamics-based gene delivery, with at least single gene transfer.

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December 2005
Volume 16 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Xiang L, Murai A and Muramatsu T: Mutated-leptin gene transfer induces increases in body weight by electroporation and hydrodynamics-based gene delivery in mice. Int J Mol Med 16: 1015-1020, 2005
APA
Xiang, L., Murai, A., & Muramatsu, T. (2005). Mutated-leptin gene transfer induces increases in body weight by electroporation and hydrodynamics-based gene delivery in mice. International Journal of Molecular Medicine, 16, 1015-1020. https://doi.org/10.3892/ijmm.16.6.1015
MLA
Xiang, L., Murai, A., Muramatsu, T."Mutated-leptin gene transfer induces increases in body weight by electroporation and hydrodynamics-based gene delivery in mice". International Journal of Molecular Medicine 16.6 (2005): 1015-1020.
Chicago
Xiang, L., Murai, A., Muramatsu, T."Mutated-leptin gene transfer induces increases in body weight by electroporation and hydrodynamics-based gene delivery in mice". International Journal of Molecular Medicine 16, no. 6 (2005): 1015-1020. https://doi.org/10.3892/ijmm.16.6.1015