The influence of Celecoxib on matrix synthesis by chondrocytes under mechanical stress in vitro

  • Authors:
    • Seiji Iimoto
    • Shohei Watanabe
    • Toshiaki Takahashi
    • Akira Shimizu
    • Haruyasu Yamamoto
  • View Affiliations

  • Published online on: December 1, 2005     https://doi.org/10.3892/ijmm.16.6.1083
  • Pages: 1083-1088
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Abstract

To assess effects of Celecoxib, selective cyclo-oxygenase (COX)-2 inhibitor, on matrix synthesis by chondrocytes under mechanical stress in vitro. Chondrocytes from 7-day-old rat articular cartilage were cultured. Cyclic mechanical stress (0.5 Hz, 7% elongation) was loaded using a Flexercell strain unit in the presence of Celecoxib, Indomethacin, and Ketoprofen. Concentrations of chondroitin sulfate (CS) in culture media were measured by high-performance liquid chromatography. Nitric oxide (NO) formation was detected by measuring NO2-accumulation in culture supernatants during the Griess reaction, using sodium nitrite as a standard. Levels of mRNA for aggrecan (AGC), type II collagen (CII), COX-2, and membrane-associated prostaglandin E synthase-1 (mPGES-1) were measured using real time reverse transcriptase-polymerase chain reaction. Under mechanical stress, levels of AGC and CII mRNA were decreased, while COX-2 mRNA levels were increased. Of the three drugs tested, only Celecoxib prevented the decrease of AGC mRNA and inhibited the release of CS. In addition, Celecoxib suppresses the level of mPGES-1 mRNA. These findings show that excessive mechanical stress decreases synthesis of matrix components in chondrocytes through a NO-regulated pathway, and suggest that Celecoxib has a protective effect on matrix metabolism.

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December 2005
Volume 16 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Iimoto, S., Watanabe, S., Takahashi, T., Shimizu, A., & Yamamoto, H. (2005). The influence of Celecoxib on matrix synthesis by chondrocytes under mechanical stress in vitro. International Journal of Molecular Medicine, 16, 1083-1088. https://doi.org/10.3892/ijmm.16.6.1083
MLA
Iimoto, S., Watanabe, S., Takahashi, T., Shimizu, A., Yamamoto, H."The influence of Celecoxib on matrix synthesis by chondrocytes under mechanical stress in vitro". International Journal of Molecular Medicine 16.6 (2005): 1083-1088.
Chicago
Iimoto, S., Watanabe, S., Takahashi, T., Shimizu, A., Yamamoto, H."The influence of Celecoxib on matrix synthesis by chondrocytes under mechanical stress in vitro". International Journal of Molecular Medicine 16, no. 6 (2005): 1083-1088. https://doi.org/10.3892/ijmm.16.6.1083