Diosgenin, a plant steroid, induces apoptosis in COX-2 deficient K562 cells with activation of the p38 MAP kinase signalling and inhibition of NF-κB binding

  • Authors:
    • Bertrand Liagre
    • Julian Bertrand
    • David Y. Leger
    • Jean-Louis Beneytout
  • View Affiliations

  • Published online on: December 1, 2005     https://doi.org/10.3892/ijmm.16.6.1095
  • Pages: 1095-1101
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Abstract

Diosgenin is a steroidal sapogenin with antitumor properties. We previously showed that diosgenin induced apoptosis in human erythroleukemia (HEL) cells. In order to elucidate the mechanism of its apoptotic activity, we investigated the effect of diosgenin on nuclear factor-κB (NF-κB) binding and on three groups of human mitogen-activated protein kinases (MAPKs) in relation to diosgenin-induced apoptosis in different erythroleukemia cell lines (K562 and HEL). Our results showed that diosgenin decreased DNA binding of NF-κB in K562 and HEL cells after 48-h diosgenin treatment. This inhibition of NF-κB binding was correlated with strong apoptosis in both erythroleukemia cell lines. Diosgenin-induced apoptosis was associated with cyclo-oxygenase-2 (COX-2) up-regulation in HEL cells but not in K562 cells which are COX-2 deficient. Furthermore, diosgenin inhibited extracellular signal-regulated kinase (ERK) activation only in HEL cells. However, diosgenin activated p38 MAPK in both cell lines and activated c-jun NH2-terminal kinases (JNKs) only in HEL cells. Pre-treatment with a selective p38 inhibitor inhibited diosgenin-induced DNA fragmentation in K562 cells. For the first time, our results suggest that inhibition of NF-κB nuclear binding and p38 MAPK activation are involved in the diosgenin-mediated signal cascades in K562 cells for inducing/regulating DNA fragmentation.

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December 2005
Volume 16 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Liagre B, Bertrand J, Leger DY and Beneytout J: Diosgenin, a plant steroid, induces apoptosis in COX-2 deficient K562 cells with activation of the p38 MAP kinase signalling and inhibition of NF-κB binding. Int J Mol Med 16: 1095-1101, 2005
APA
Liagre, B., Bertrand, J., Leger, D.Y., & Beneytout, J. (2005). Diosgenin, a plant steroid, induces apoptosis in COX-2 deficient K562 cells with activation of the p38 MAP kinase signalling and inhibition of NF-κB binding. International Journal of Molecular Medicine, 16, 1095-1101. https://doi.org/10.3892/ijmm.16.6.1095
MLA
Liagre, B., Bertrand, J., Leger, D. Y., Beneytout, J."Diosgenin, a plant steroid, induces apoptosis in COX-2 deficient K562 cells with activation of the p38 MAP kinase signalling and inhibition of NF-κB binding". International Journal of Molecular Medicine 16.6 (2005): 1095-1101.
Chicago
Liagre, B., Bertrand, J., Leger, D. Y., Beneytout, J."Diosgenin, a plant steroid, induces apoptosis in COX-2 deficient K562 cells with activation of the p38 MAP kinase signalling and inhibition of NF-κB binding". International Journal of Molecular Medicine 16, no. 6 (2005): 1095-1101. https://doi.org/10.3892/ijmm.16.6.1095