The calcium-sensing receptor and vitamin D receptor expression in tertiary hyperparathyroidism

  • Authors:
    • Tomasz Grzela
    • Witold Chudzinski
    • Zofia Lasiecka
    • Justyna Niderla
    • Grzegorz Wilczynski
    • Barbara Gornicka
    • Aleksander Wasiutynski
    • Magdalena Durlik
    • Anna Boszczyk
    • Robert Brawura-Biskupski-Samaha
    • Piotr Dziunycz
    • Lukasz Milewski
    • Marta Lazarczyk
    • Maciej Lazarczyk
    • Ireneusz Nawrot
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijmm.17.5.779
  • Pages: 779-783
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Abstract

The parathormone (PTH) production is controlled by calcium and vitamin D, which interact with the calcium-sensing receptor (CaSR) and vitamin D receptor (VDR), respectively. All of these elements control calcium homeostasis, which is crucial for many physiological processes. Thus, impairment of the upstream component of this system, e.g. a decrease of CaSR and/or VDR, could result in hyperparathyroidism (HPTH). Therefore, the aim of this study was to assess the expression of CaSR and VDR in a tertiary form of HPTH (T-HPTH). The study involved 19 T-HPTH patients qualified for parathyroidectomy and 21 control parathyroids harvested from multi-organ cadaver donors. The small fragments of harvested glands were homogenized and used for Western blot analysis, whereas the remaining tissues underwent routine hematoxylin-eosin staining or immunostaining for CaSR and VDR. Among 64 T-HPTH parathyroids, 58 revealed the morphology of benign hyperplasia, 2 were identified as adenoma and 4 were classified as normal; some glands displayed a mixed histological phenotype. Western blot analysis revealed a decrease of CaSR and VDR in hyperplasia and adenoma-derived samples. However, no correlation between the types of hyperplasia and receptor expression was observed. On the other hand, microscopic analysis of CaSR- and VDR-immunostained sections revealed a highly differentiated and significantly decreased mean expression of both receptors, which correlated with parathyroid histology. The reason behind the impaired expression of CaSR and VDR in T-HPTH is unclear. It presumably results from constant parathyroid stimulation at the stage of S-HPTH, followed by further development of polyclonal autonomy. However, the verification of this thesis requires further study.

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May 2006
Volume 17 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Grzela T, Chudzinski W, Lasiecka Z, Niderla J, Wilczynski G, Gornicka B, Wasiutynski A, Durlik M, Boszczyk A, Brawura-Biskupski-Samaha R, Brawura-Biskupski-Samaha R, et al: The calcium-sensing receptor and vitamin D receptor expression in tertiary hyperparathyroidism. Int J Mol Med 17: 779-783, 2006.
APA
Grzela, T., Chudzinski, W., Lasiecka, Z., Niderla, J., Wilczynski, G., Gornicka, B. ... Nawrot, I. (2006). The calcium-sensing receptor and vitamin D receptor expression in tertiary hyperparathyroidism. International Journal of Molecular Medicine, 17, 779-783. https://doi.org/10.3892/ijmm.17.5.779
MLA
Grzela, T., Chudzinski, W., Lasiecka, Z., Niderla, J., Wilczynski, G., Gornicka, B., Wasiutynski, A., Durlik, M., Boszczyk, A., Brawura-Biskupski-Samaha, R., Dziunycz, P., Milewski, L., Lazarczyk, M., Lazarczyk, M., Nawrot, I."The calcium-sensing receptor and vitamin D receptor expression in tertiary hyperparathyroidism". International Journal of Molecular Medicine 17.5 (2006): 779-783.
Chicago
Grzela, T., Chudzinski, W., Lasiecka, Z., Niderla, J., Wilczynski, G., Gornicka, B., Wasiutynski, A., Durlik, M., Boszczyk, A., Brawura-Biskupski-Samaha, R., Dziunycz, P., Milewski, L., Lazarczyk, M., Lazarczyk, M., Nawrot, I."The calcium-sensing receptor and vitamin D receptor expression in tertiary hyperparathyroidism". International Journal of Molecular Medicine 17, no. 5 (2006): 779-783. https://doi.org/10.3892/ijmm.17.5.779