Accumulation of steroidogenic acute regulatory protein mRNA, and decrease in the secretory and proliferative activity of rat adrenocortical cells in the presence of proteasome inhibitors

  • Authors:
    • Agnieska Ziolkowska
    • Cinzia Tortorella
    • Gastone G. Nussdorfer
    • Marcin Rucinski
    • Mariola Majchrzak
    • Ludwik K. Malendowicz
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijmm.17.5.865
  • Pages: 865-868
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Abstract

Sporadic findings indicate that proteolysis may affect steroid secretion in rat ovary granulosa cells. We examined the effects of the proteasome inhibitors MG115 and MG101 on the in vitro secretion and growth of rat adrenocortical cells. MG115 and/or MG101 decreased within 120 min the aldosterone and corticosterone secretion from freshly dispersed zona glomerulosa and zona fasciculata-reticularis (ZF/R) cells. After a 24-h incubation MG115 alone or with MG101 lowered corticosterone production and enhanced proliferation rate of cultured ZF/R cells, while MG101 was per se ineffective. Real-time polymerase chain reaction demonstrated that MG101 decreased steroidogenic acute regulatory protein (StAR) mRNA in cultured cells. MG115 was per se ineffective, but when added together with MG101 evoked a marked rise in StAR mRNA content. In light of the present findings, we conclude that i) protein breackdown by proteasomes is required for the maintenance of a normal secretory and proliferative activity of freshly dispersed or cultured rat adrenocortical cells; and ii) in long-term experiments, great caution must be taken in correlating StAR mRNA content and steroidogenic capacity.

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May 2006
Volume 17 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ziolkowska A, Tortorella C, Nussdorfer GG, Rucinski M, Majchrzak M and Malendowicz LK: Accumulation of steroidogenic acute regulatory protein mRNA, and decrease in the secretory and proliferative activity of rat adrenocortical cells in the presence of proteasome inhibitors. Int J Mol Med 17: 865-868, 2006
APA
Ziolkowska, A., Tortorella, C., Nussdorfer, G.G., Rucinski, M., Majchrzak, M., & Malendowicz, L.K. (2006). Accumulation of steroidogenic acute regulatory protein mRNA, and decrease in the secretory and proliferative activity of rat adrenocortical cells in the presence of proteasome inhibitors. International Journal of Molecular Medicine, 17, 865-868. https://doi.org/10.3892/ijmm.17.5.865
MLA
Ziolkowska, A., Tortorella, C., Nussdorfer, G. G., Rucinski, M., Majchrzak, M., Malendowicz, L. K."Accumulation of steroidogenic acute regulatory protein mRNA, and decrease in the secretory and proliferative activity of rat adrenocortical cells in the presence of proteasome inhibitors". International Journal of Molecular Medicine 17.5 (2006): 865-868.
Chicago
Ziolkowska, A., Tortorella, C., Nussdorfer, G. G., Rucinski, M., Majchrzak, M., Malendowicz, L. K."Accumulation of steroidogenic acute regulatory protein mRNA, and decrease in the secretory and proliferative activity of rat adrenocortical cells in the presence of proteasome inhibitors". International Journal of Molecular Medicine 17, no. 5 (2006): 865-868. https://doi.org/10.3892/ijmm.17.5.865