Abnormal DNA end-joining activity in human head and neck cancer

  • Authors:
    • Ki-Hyuk Shin
    • Mo K. Kang
    • Reuben H. Kim
    • Ayako Kameta
    • Marcel A. Baluda
    • No-Hee Park
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijmm.17.5.917
  • Pages: 917-924
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Abstract

In human cells, DNA double-strand breaks (DSBs) are repaired primarily by the DNA end-joining (EJ) process and thus, abnormal DNA EJ activities lead to an accumulation of mutations and/or aneuploidy, resulting in genetic instability of cells. Since genetic instability is the hallmark of cancer cells, we studied the DNA EJ activities of normal, non-malignant immortalized and malignant human epithelial cells to investigate the association between DNA EJ and carcinogenesis. We found a significant diminution of precise (error-free) DNA EJ activities in non-malignant immortalized human oral keratinocytes (HOK-16B) and human head and neck squamous cell carcinoma (HNSCC) cells compared to that in normal human oral keratinocytes (NHOK). Moreover, abnormal DNA EJ activities were detected exclusively in HOK-16B and HNSCC cells due to microhomology-mediated and non-microhomology-mediated end-joining activities in these cells. These data indicated that aberrant DNA EJ activity may be partly responsible for genetic instability and oncogenic transformation.

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May 2006
Volume 17 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shin K, Kang MK, Kim RH, Kameta A, Baluda MA and Park N: Abnormal DNA end-joining activity in human head and neck cancer. Int J Mol Med 17: 917-924, 2006
APA
Shin, K., Kang, M.K., Kim, R.H., Kameta, A., Baluda, M.A., & Park, N. (2006). Abnormal DNA end-joining activity in human head and neck cancer. International Journal of Molecular Medicine, 17, 917-924. https://doi.org/10.3892/ijmm.17.5.917
MLA
Shin, K., Kang, M. K., Kim, R. H., Kameta, A., Baluda, M. A., Park, N."Abnormal DNA end-joining activity in human head and neck cancer". International Journal of Molecular Medicine 17.5 (2006): 917-924.
Chicago
Shin, K., Kang, M. K., Kim, R. H., Kameta, A., Baluda, M. A., Park, N."Abnormal DNA end-joining activity in human head and neck cancer". International Journal of Molecular Medicine 17, no. 5 (2006): 917-924. https://doi.org/10.3892/ijmm.17.5.917