Molecular basis for the involvement of thymidine phosphorylase in cancer invasion

  • Authors:
    • Takenari Gotanda
    • Misako Haraguchi
    • Tokushi Tachiwada
    • Reiko Shinkura
    • Chihaya Koriyama
    • Suminori Akiba
    • Motoshi Kawahara
    • Kenryu Nishiyama
    • Tomoyuki Sumizawa
    • Tatsuhiko Furukawa
    • Hiromitsu Mimata
    • Yoshio Nomura
    • Shin-ichi Akiyama
    • Masayuki Nakagawa
  • View Affiliations

  • Published online on: June 1, 2006     https://doi.org/10.3892/ijmm.17.6.1085
  • Pages: 1085-1091
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Abstract

Thymidine phosphorylase (TP), also known as platelet-derived endothelial cell growth factor (PD-ECGF), has been implicated in bladder cancer angiogenesis and invasion. However, the molecular basis of its role in invasion remains unclear. We investigated the expression of TP and 10 invasion-related genes in bladder cancers from 72 randomly selected patients by real-time two-step RT-PCR assay. We found that the expression levels of TP, MMP-9, uPA, and MMP-2 were significantly higher in invasive tumors than those in superficial tumors. Also, the expression level of TP significantly correlated with that of uPA, MMP-1, MMP-9, PAI-1 and VEGF. KK47/TP cells, bladder cancer cells that overexpress TP, had a higher expression of MMP-7 and MMP-9 than KK/CV cells that express lower level of TP in hypoxic condition. PC/TP cells, prostate cancer cells that overexpress TP, also had a higher expression of MMP-1 and MMP-7 than PC/CV cells that express no detectable TP. Taken together these data indicate that TP enhances the invasion of tumor cells through the induction of invasion-related genes.

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June 2006
Volume 17 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Gotanda T, Haraguchi M, Tachiwada T, Shinkura R, Koriyama C, Akiba S, Kawahara M, Nishiyama K, Sumizawa T, Furukawa T, Furukawa T, et al: Molecular basis for the involvement of thymidine phosphorylase in cancer invasion. Int J Mol Med 17: 1085-1091, 2006.
APA
Gotanda, T., Haraguchi, M., Tachiwada, T., Shinkura, R., Koriyama, C., Akiba, S. ... Nakagawa, M. (2006). Molecular basis for the involvement of thymidine phosphorylase in cancer invasion. International Journal of Molecular Medicine, 17, 1085-1091. https://doi.org/10.3892/ijmm.17.6.1085
MLA
Gotanda, T., Haraguchi, M., Tachiwada, T., Shinkura, R., Koriyama, C., Akiba, S., Kawahara, M., Nishiyama, K., Sumizawa, T., Furukawa, T., Mimata, H., Nomura, Y., Akiyama, S., Nakagawa, M."Molecular basis for the involvement of thymidine phosphorylase in cancer invasion". International Journal of Molecular Medicine 17.6 (2006): 1085-1091.
Chicago
Gotanda, T., Haraguchi, M., Tachiwada, T., Shinkura, R., Koriyama, C., Akiba, S., Kawahara, M., Nishiyama, K., Sumizawa, T., Furukawa, T., Mimata, H., Nomura, Y., Akiyama, S., Nakagawa, M."Molecular basis for the involvement of thymidine phosphorylase in cancer invasion". International Journal of Molecular Medicine 17, no. 6 (2006): 1085-1091. https://doi.org/10.3892/ijmm.17.6.1085