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Characterization of a novel human protein phosphatase 2C family member, PP2Cκ

  • Authors:
    • Jianfeng Dai
    • Jiyuan Zhang
    • Yaqiong Sun
    • Qihan Wu
    • Liyun Sun
    • Chaoneng Ji
    • Shaohua Gu
    • Congjing Feng
    • Yi Xie
    • Yumin Mao

  • View Affiliations

  • Published online on: June 1, 2006     https://doi.org/10.3892/ijmm.17.6.1117
  • Pages: 1117-1123
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

A novel member of human protein phosphatase 2C gene named PP2Ck was isolated from a human fetal brain cDNA library. The 2.0 kb cDNA encodes a 372 amino acid polypeptide with an intact protein phosphatase 2C (PP2C) catalytic domain. Reverse transcription-PCR (RT-PCR) revealed that the PP2Cκ was widely expressed in normal human tissues. Transient transfection suggested that PP2Cκ was localized in the nucleus in AD293 cells. Recombinant Trx-His-PP2Cκ showed phosphatase activity toward p-nitrophenyl phosphate (pNPP), as well as oligopeptides containing phospho-threonine residues. Furthermore, the overexpression of PP2Cκ distinctly activated the heat shock transcription factor pathway in eukaryotic cells.

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Journal Cover

June 2006
Volume 17 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Dai J, Zhang J, Sun Y, Wu Q, Sun L, Ji C, Gu S, Feng C, Xie Y, Mao Y, Mao Y, et al: Characterization of a novel human protein phosphatase 2C family member, PP2Cκ. Int J Mol Med 17: 1117-1123, 2006
APA
Dai, J., Zhang, J., Sun, Y., Wu, Q., Sun, L., Ji, C. ... Mao, Y. (2006). Characterization of a novel human protein phosphatase 2C family member, PP2Cκ. International Journal of Molecular Medicine, 17, 1117-1123. https://doi.org/10.3892/ijmm.17.6.1117
MLA
Dai, J., Zhang, J., Sun, Y., Wu, Q., Sun, L., Ji, C., Gu, S., Feng, C., Xie, Y., Mao, Y."Characterization of a novel human protein phosphatase 2C family member, PP2Cκ". International Journal of Molecular Medicine 17.6 (2006): 1117-1123.
Chicago
Dai, J., Zhang, J., Sun, Y., Wu, Q., Sun, L., Ji, C., Gu, S., Feng, C., Xie, Y., Mao, Y."Characterization of a novel human protein phosphatase 2C family member, PP2Cκ". International Journal of Molecular Medicine 17, no. 6 (2006): 1117-1123. https://doi.org/10.3892/ijmm.17.6.1117