Effect of GLP-1 on D-glucose transport, lipolysis and lipogenesis in adipocytes of obese subjects

  • Authors:
    • Verónica Sancho
    • María V. Trigo
    • Antonio Martín-Duce
    • Nieves González
    • Alicia Acitores
    • Luis Arnés
    • Isabel Valverde
    • Willy J. Malaisse
    • María L. Villanueva-Peñacarrillo
  • View Affiliations

  • Published online on: June 1, 2006     https://doi.org/10.3892/ijmm.17.6.1133
  • Pages: 1133-1137
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Abstract

GLP-1 has anorectic properties and regulates fuel homeostasis through both its insulinotropic and insulinotrophic actions and effects in extrapancreatic tissue. This study is aimed at characterizing the response to GLP-1 of adipocytes from obese patients, in terms of D-glucose transport and lipid metabolism, in comparison with data from normal subjects. Adipocytes were obtained by enzymatic digestion from the abdominal fat tissue of 25 morbidly obese patients and 8 normal subjects undergoing bariatric or inguinal hernia surgery, respectively. Basal GLUT4 expression, D-glucose transport, glycerol release and lipogenesis were measured in cells treated, when required, with 10−12-10−9 M GLP-1, insulin, glucagon and the GLP-1 structurally related peptides, exendin-4 and exendin-9. In obese patients, versus normal subjects, a trend towards lower values was found in GLUT4 protein or mRNA, although the differences were not statistically significant; insulin-stimulated glucose uptake was higher and cells did not respond to GLP-1, while both exendins (10−10 and 10−9 M) exerted an inhibitory action; basal lipolysis was higher and so was the effect of GLP-1 and glucagon, whereas insulin abolished the lipolytic action of all peptides; both basal lipogenesis and the response to insulin were higher while GLP-1 and exendin-4 were ineffective. These results document the analogies and dissimilarities between the response to GLP-1, exendin-4 and exendin-9, as well as to insulin and glucagon, relative to glucose transport and lipid metabolism of fat tissue from obese patients versus normal subjects, the reduced lipogenic effect and enhanced lipolytic action of GLP-1 being, perhaps, adequate for its therapeutic use in obesity.

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June 2006
Volume 17 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sancho V, Trigo MV, Martín-Duce A, González N, Acitores A, Arnés L, Valverde I, Malaisse WJ and Villanueva-Peñacarrillo ML: Effect of GLP-1 on D-glucose transport, lipolysis and lipogenesis in adipocytes of obese subjects. Int J Mol Med 17: 1133-1137, 2006
APA
Sancho, V., Trigo, M.V., Martín-Duce, A., González, N., Acitores, A., Arnés, L. ... Villanueva-Peñacarrillo, M.L. (2006). Effect of GLP-1 on D-glucose transport, lipolysis and lipogenesis in adipocytes of obese subjects. International Journal of Molecular Medicine, 17, 1133-1137. https://doi.org/10.3892/ijmm.17.6.1133
MLA
Sancho, V., Trigo, M. V., Martín-Duce, A., González, N., Acitores, A., Arnés, L., Valverde, I., Malaisse, W. J., Villanueva-Peñacarrillo, M. L."Effect of GLP-1 on D-glucose transport, lipolysis and lipogenesis in adipocytes of obese subjects". International Journal of Molecular Medicine 17.6 (2006): 1133-1137.
Chicago
Sancho, V., Trigo, M. V., Martín-Duce, A., González, N., Acitores, A., Arnés, L., Valverde, I., Malaisse, W. J., Villanueva-Peñacarrillo, M. L."Effect of GLP-1 on D-glucose transport, lipolysis and lipogenesis in adipocytes of obese subjects". International Journal of Molecular Medicine 17, no. 6 (2006): 1133-1137. https://doi.org/10.3892/ijmm.17.6.1133