Desflurane differentially affects the release of proinflammatory cytokines in plasma and bronchoalveolar fluid of endotoxemic rats

  • Authors:
    • Kim A. Boost
    • Christian Hofstetter
    • Michael Flondor
    • Christian Betz
    • Markus Homann
    • Josef Pfeilschifter
    • Heiko Muehl
    • Bernhard Zwissler
  • View Affiliations

  • Published online on: June 1, 2006     https://doi.org/10.3892/ijmm.17.6.1139
  • Pages: 1139-1144
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Abstract

Previous studies have indicated that volatile anaesthetics can attenuate the inflammatory response to lipopolysaccharide (LPS) and other proinflammatory stimuli in vitro and in vivo. Thus far, no studies are available on the influences of desflurane on the cytokine-release. We therefore aimed to investigate the effects of desflurane on the systemic and pulmonary release of proinflammatory cytokines in endotoxemic rats. Eighteen anaesthetized and ventilated Sprague-Dawley rats were randomly assigned to the following groups: LPS-only: Six animals received LPS (5 mg/kg, i.v.) with no further intervention. LPS-Desflurane: Six animals received continuous inhalation of 1MAC Desflurane before and during endotoxemia with LPS (5 mg/kg, i.v.). Sham: Six animals served as control without inhalation of desflurane and endotoxemia. After 4 h, levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in plasma and bronchoalveolar fluid were analyzed. Nitrite production as a readout for nitric oxide (NO) release from alveolar macrophages was measured by Griess assay. IκB-α degradation and iNOS-protein in macrophage homogenates were determined by Western Blotting. Inhalation of desflurane during endotoxemia showed a significant decrease in release of the proinflammatory cytokines TNF-α (−61%, P≤0.05) and IL-1β (−47%, P≤0.05) in plasma as compared to LPS-only group, whereas the release of IL-6 was not significantly affected by desflurane. Within the lung, the NO-release was notably increased in supernatants of cultured alveolar macrophages from desflurane-group compared to both LPS-only and Sham group. IκB-α degradation in alveolar macrophages was impaired in the Desflurane-group as compared to the LPS-only group. Our data implicate that inhalation of 1MAC Desflurane during experimental endotoxemia differentially affects the inflammatory response in rats.

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June 2006
Volume 17 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Boost KA, Hofstetter C, Flondor M, Betz C, Homann M, Pfeilschifter J, Muehl H and Zwissler B: Desflurane differentially affects the release of proinflammatory cytokines in plasma and bronchoalveolar fluid of endotoxemic rats. Int J Mol Med 17: 1139-1144, 2006.
APA
Boost, K.A., Hofstetter, C., Flondor, M., Betz, C., Homann, M., Pfeilschifter, J. ... Zwissler, B. (2006). Desflurane differentially affects the release of proinflammatory cytokines in plasma and bronchoalveolar fluid of endotoxemic rats. International Journal of Molecular Medicine, 17, 1139-1144. https://doi.org/10.3892/ijmm.17.6.1139
MLA
Boost, K. A., Hofstetter, C., Flondor, M., Betz, C., Homann, M., Pfeilschifter, J., Muehl, H., Zwissler, B."Desflurane differentially affects the release of proinflammatory cytokines in plasma and bronchoalveolar fluid of endotoxemic rats". International Journal of Molecular Medicine 17.6 (2006): 1139-1144.
Chicago
Boost, K. A., Hofstetter, C., Flondor, M., Betz, C., Homann, M., Pfeilschifter, J., Muehl, H., Zwissler, B."Desflurane differentially affects the release of proinflammatory cytokines in plasma and bronchoalveolar fluid of endotoxemic rats". International Journal of Molecular Medicine 17, no. 6 (2006): 1139-1144. https://doi.org/10.3892/ijmm.17.6.1139