Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells

  • Authors:
    • Elisa Callegari
    • Giuseppe D. Norata
    • Hiroyasu Inoue
    • Alberico L. Catapano
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/ijmm.18.1.209
  • Pages: 209-213
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Abstract

Modified high density lipoprotein (HDL) has been suggested to modulate endothelial expression of pro-inflammatory genes. Since oxidised HDL (Ox-HDL) has been found in atheromatous plaques and receptors for modified HDL are present on endothelial cells, we investigated the effect of Ox-HDL3 on the expression of Cox-1 or Cox-2. Ox-HDL3, increased Cox-2 mRNA and protein expression in endothelial cells while no effect on Cox-1 expression was observed. The intracellular pathways involved in this effect were investigated. The incubation with specific inhibitors of intracellular kinases showed that PI3K is mainly involved in the Ox-HDL3-dependent Cox-2 induction. Transient transfection experiments suggested that the NF-IL6 response element in the proximal promoter (-327 to 59) is involved in Ox-HDL3-mediated Cox-2 expression. These data suggest that Ox-HDL induce Cox-2 expression in endothelial cells through a PI3K/NF-IL6-dependent pathway.

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July 2006
Volume 18 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Callegari E, Norata GD, Inoue H and Catapano AL: Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells. Int J Mol Med 18: 209-213, 2006
APA
Callegari, E., Norata, G.D., Inoue, H., & Catapano, A.L. (2006). Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells. International Journal of Molecular Medicine, 18, 209-213. https://doi.org/10.3892/ijmm.18.1.209
MLA
Callegari, E., Norata, G. D., Inoue, H., Catapano, A. L."Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells". International Journal of Molecular Medicine 18.1 (2006): 209-213.
Chicago
Callegari, E., Norata, G. D., Inoue, H., Catapano, A. L."Oxidized-HDL3 modulates the expression of Cox-2 in human endothelial cells". International Journal of Molecular Medicine 18, no. 1 (2006): 209-213. https://doi.org/10.3892/ijmm.18.1.209