Endothelial cells from human cerebral aneurysm and arteriovenous malformation release ET-1 in response to vessel rupture

  • Authors:
    • Elisa Boscolo
    • Giacomo Pavesi
    • Paolo Zampieri
    • Maria Teresa Conconi
    • Chiara Calore
    • Renato Scienza
    • Pier Paolo Parnigotto
    • Marcella Folin
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijmm.18.5.813
  • Pages: 813-819
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Abstract

Cerebral aneurysms and arteriovenous malformations (AVM) are a common cause of stroke and cerebral hemorrage. Both are often discovered when they rupture, causing subarachnoid hemorrhage (SAH). SAH-induced vasospasm is mediated by enhanced vasoconstriction due to endothelin-1 (ET-1). We investigated whether endothelial cells (ECs) obtained from aneurysm and AVM express phenotypic and genotypic alterations contributing to the development of vasospasm after SAH. We isolated ECs from human AVM and aneurysm and then confirmed their EC origin by polymerase chain reaction and immunocytochemistry with endothelial markers. Experiments were also carried out with human cerebral microvascular and umbilical vein ECs (HCECs and HUVECs respectively) for comparison. We tested EC proliferation ability and microtubule formation in Matrigel at different cell passages. Five aneurysm (3 ruptured, 2 unruptured) and 3 AVM (2 ruptured, 1 unruptured) ECs were tested for ET-1 release in the culture medium. Aneurysm and AVM ECs expressed von Willebrand factor, Adrenomedullin, and exhibited a progressive reduction of proliferation and in vitro angiogenic ability after the V passage. Significantly higher levels of ET-1 have been detected in ECs from ruptured aneurysms and AVMs. We report the first successful isolation and characterization of primary EC lines from human cerebral vascular lesions. Augmented release of ET-1 is correlated with the rupture of the abnormal vessel confirming its role in vasospasm after SAH. Furthermore, ECs obtained from these vascular malformations can be used as an experimental model to study SAH-induced vasoconstriction.

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November 2006
Volume 18 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Boscolo E, Pavesi G, Zampieri P, Conconi MT, Calore C, Scienza R, Parnigotto PP and Folin M: Endothelial cells from human cerebral aneurysm and arteriovenous malformation release ET-1 in response to vessel rupture. Int J Mol Med 18: 813-819, 2006
APA
Boscolo, E., Pavesi, G., Zampieri, P., Conconi, M.T., Calore, C., Scienza, R. ... Folin, M. (2006). Endothelial cells from human cerebral aneurysm and arteriovenous malformation release ET-1 in response to vessel rupture. International Journal of Molecular Medicine, 18, 813-819. https://doi.org/10.3892/ijmm.18.5.813
MLA
Boscolo, E., Pavesi, G., Zampieri, P., Conconi, M. T., Calore, C., Scienza, R., Parnigotto, P. P., Folin, M."Endothelial cells from human cerebral aneurysm and arteriovenous malformation release ET-1 in response to vessel rupture". International Journal of Molecular Medicine 18.5 (2006): 813-819.
Chicago
Boscolo, E., Pavesi, G., Zampieri, P., Conconi, M. T., Calore, C., Scienza, R., Parnigotto, P. P., Folin, M."Endothelial cells from human cerebral aneurysm and arteriovenous malformation release ET-1 in response to vessel rupture". International Journal of Molecular Medicine 18, no. 5 (2006): 813-819. https://doi.org/10.3892/ijmm.18.5.813