Neuromedin U receptor 1 expression in the rat endocrine pancreas and evidence suggesting neuromedin U suppressive effect on insulin secretion from isolated rat pancreatic islets

  • Authors:
    • Przemyslaw Kaczmarek
    • Ludwik K. Malendowicz
    • Ewa Pruszynska-Oszmalek
    • Tatiana Wojciechowicz
    • Dawid Szczepankiewicz
    • Tomasz Szkudelski
    • Krzysztof W. Nowak
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijmm.18.5.951
  • Pages: 951-955
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Abstract

Neuromedin U (NmU) is a regulatory peptide found in significant concentrations in both the brain and gut of the rat and is named according to its ability to powerfully contract the uterus. Two types of NmU receptors were recently identified and subsequent studies evidenced NmU involvement in the regulation of energy homeostasis. Such a role of neuromedin U suggests that a polypeptide may also be involved in the regulation of adipoinsular axis function. Therefore in the present study we examined the expression of NmU receptors in pancreatic islets using RT-PCR and Western blotting analysis. We also investigated the role of NmU in regulation of insulin secretion in vitro using isolated pancreatic islets. We have confirmed that NmUR1 but not NmUR2 is specifically expressed in isolated rat pancreatic islets. In all tested doses (1, 10, 100 nmol/l) NmU dose- dependently decreased insulin output by isolated pancreatic islets. These inhibitory effects of NmU on insulin secretion may suggest the involvement of NmU in regulating the pancreatic branch of adipoinsular axis function. Thus, NmU can be included in that group of anorectic peptides, which are also involved in the regulation of insulin secretion.

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November 2006
Volume 18 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kaczmarek P, Malendowicz LK, Pruszynska-Oszmalek E, Wojciechowicz T, Szczepankiewicz D, Szkudelski T and Nowak KW: Neuromedin U receptor 1 expression in the rat endocrine pancreas and evidence suggesting neuromedin U suppressive effect on insulin secretion from isolated rat pancreatic islets. Int J Mol Med 18: 951-955, 2006
APA
Kaczmarek, P., Malendowicz, L.K., Pruszynska-Oszmalek, E., Wojciechowicz, T., Szczepankiewicz, D., Szkudelski, T., & Nowak, K.W. (2006). Neuromedin U receptor 1 expression in the rat endocrine pancreas and evidence suggesting neuromedin U suppressive effect on insulin secretion from isolated rat pancreatic islets. International Journal of Molecular Medicine, 18, 951-955. https://doi.org/10.3892/ijmm.18.5.951
MLA
Kaczmarek, P., Malendowicz, L. K., Pruszynska-Oszmalek, E., Wojciechowicz, T., Szczepankiewicz, D., Szkudelski, T., Nowak, K. W."Neuromedin U receptor 1 expression in the rat endocrine pancreas and evidence suggesting neuromedin U suppressive effect on insulin secretion from isolated rat pancreatic islets". International Journal of Molecular Medicine 18.5 (2006): 951-955.
Chicago
Kaczmarek, P., Malendowicz, L. K., Pruszynska-Oszmalek, E., Wojciechowicz, T., Szczepankiewicz, D., Szkudelski, T., Nowak, K. W."Neuromedin U receptor 1 expression in the rat endocrine pancreas and evidence suggesting neuromedin U suppressive effect on insulin secretion from isolated rat pancreatic islets". International Journal of Molecular Medicine 18, no. 5 (2006): 951-955. https://doi.org/10.3892/ijmm.18.5.951