Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro

  • Authors:
    • Raffaella Spinazzi
    • Giovanna Albertin
    • Beatrice Nico
    • Diego Guidolin
    • Rosa Di Liddo
    • Gian Paolo Rossi
    • Domenico Ribatti
    • Gastone G. Nussdorfer
  • View Affiliations

  • Published online on: December 1, 2006     https://doi.org/10.3892/ijmm.18.6.1107
  • Pages: 1107-1112
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Abstract

Urotensin-II (UII), along its receptor UT-R, is widely expressed in the cardiovascular system, where it exerts regulatory actions under both physiological and pathological conditions. Real-time PCR and immunocytochemistry demonstrated the expression of UII and UT-R as mRNA and protein in rat neuromicrovascular endothelial cells (NECs). UII did not affect the proliferation rate of cultured NECs, but exerted a strong angiogenic action in both an in vitro assay on Matrigel and an in vivo assay on chorioallantoic membrane. The angiogenic effect of UII was similar to that of FGF-2, and was abolished by the UT-R antagonist Palosuran. Collectively, our findings allow us to include UII in the group of cytokines (e.g. endothelin-1 and adrenomedullin), which are expressed in endothelial cells and exert a pro-angiogenic effect acting in an autocrine-paracrine manner.

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December 2006
Volume 18 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Spinazzi R, Albertin G, Nico B, Guidolin D, Di Liddo R, Rossi GP, Ribatti D and Nussdorfer GG: Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro. Int J Mol Med 18: 1107-1112, 2006
APA
Spinazzi, R., Albertin, G., Nico, B., Guidolin, D., Di Liddo, R., Rossi, G.P. ... Nussdorfer, G.G. (2006). Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro. International Journal of Molecular Medicine, 18, 1107-1112. https://doi.org/10.3892/ijmm.18.6.1107
MLA
Spinazzi, R., Albertin, G., Nico, B., Guidolin, D., Di Liddo, R., Rossi, G. P., Ribatti, D., Nussdorfer, G. G."Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro". International Journal of Molecular Medicine 18.6 (2006): 1107-1112.
Chicago
Spinazzi, R., Albertin, G., Nico, B., Guidolin, D., Di Liddo, R., Rossi, G. P., Ribatti, D., Nussdorfer, G. G."Urotensin-II and its receptor (UT-R) are expressed in rat brain endothelial cells, and urotensin-II via UT-R stimulates angiogenesis in vivo and in vitro". International Journal of Molecular Medicine 18, no. 6 (2006): 1107-1112. https://doi.org/10.3892/ijmm.18.6.1107