Fibroblasts mediate induction of high mobility group box protein 1 in lung epithelial cancer cells by diffusible factors

  • Authors:
    • Babett Bartling
    • Christine Fuchs
    • Rolf-Edgar Silber
    • Andreas Simm
  • View Affiliations

  • Published online on: August 1, 2007     https://doi.org/10.3892/ijmm.20.2.217
  • Pages: 217-224
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cancer development is associated with the high mobility group box protein 1 (HMGB1), which modulates the transcriptional activity in the nucleus, but it is also present in the cytoplasm and outside the cell in certain conditions. As the progression of lung cancer is supported by mitogenic stimuli of stromal fibroblasts, we studied the impact of lung fibroblasts (WI-38) on the expression and localization of HMGB1 in lung epithelial cancer cells (H358). HMGB1 was mainly localized in the nucleus of non-mitotic H358 cells but highly distributed in the cytoplasm of mitotic cells. Conditioned medium (CM) from WI-38 fibroblasts enhanced the expression of HMGB1 at the mRNA and protein level compared to the control CM from H358 cells. In particular, the amount of cytoplasmic HMGB1 was elevated in response to fibroblast CM, which was reduced by inhibiting the basic fibroblast growth factor with blocking antibodies. Although cytoplasmic HMGB1 can be released from the cell, we did not determine a significant amount of extracellular HMGB1 in these conditions. This might partially be based on the sensitivity of HMGB1 to extracellular proteases as CM caused fast proteolysis of the cytoplasmic HMGB1 preparations. Our data suggest that diffusible factors of fibroblasts support the biological function of cancer cells via HMGB1 activation.

Related Articles

Journal Cover

August 2007
Volume 20 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Bartling B, Fuchs C, Silber R and Simm A: Fibroblasts mediate induction of high mobility group box protein 1 in lung epithelial cancer cells by diffusible factors. Int J Mol Med 20: 217-224, 2007.
APA
Bartling, B., Fuchs, C., Silber, R., & Simm, A. (2007). Fibroblasts mediate induction of high mobility group box protein 1 in lung epithelial cancer cells by diffusible factors. International Journal of Molecular Medicine, 20, 217-224. https://doi.org/10.3892/ijmm.20.2.217
MLA
Bartling, B., Fuchs, C., Silber, R., Simm, A."Fibroblasts mediate induction of high mobility group box protein 1 in lung epithelial cancer cells by diffusible factors". International Journal of Molecular Medicine 20.2 (2007): 217-224.
Chicago
Bartling, B., Fuchs, C., Silber, R., Simm, A."Fibroblasts mediate induction of high mobility group box protein 1 in lung epithelial cancer cells by diffusible factors". International Journal of Molecular Medicine 20, no. 2 (2007): 217-224. https://doi.org/10.3892/ijmm.20.2.217