Overexpression of human selenoprotein M differentially regulates the concentrations of antioxidants and H2O2, the activity of antioxidant enzymes, and the composition of white blood cells in a transgenic rat

  • Authors:
    • Dae Youn Hwang
    • Ji Soon Sin
    • Min Sun Kim
    • Su Youn Yim
    • Yong Kyu Kim
    • Chuel Kyu Kim
    • Byoung Guk Kim
    • Sun Bo Shim
    • Seung Wan Jee
    • Su Hae Lee
    • Chang Joon Bae
    • Byoung Chun Lee
    • Mee Kyung Jang
    • Jung Sik Cho
    • Kab Ryong Chae
  • View Affiliations

  • Published online on: February 1, 2008     https://doi.org/10.3892/ijmm.21.2.169
  • Pages: 169-179
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Abstract

Selenoprotein is associated with a variety of serious diseases, including infectious diseases, neurodegenerative disorders, cancer and cardiovascular disease. The aim of this study was to produce a new transgenic (Tg) rat expressing human selenoprotein M (SelM) in order to examine the protective function of the antioxidant status in vivo. To achieve this, a new lineage of Tg rats was produced by the microinjection of pCMV/GFP-hSelM constructs into a fertilized rat egg. Several conclusions can be drawn based on the results of the present study. The human SelM gene was successfully expressed at both the transcription and protein levels in the CMV/GFP-hSelM Tg rats. This Tg rat showed a different enzyme activity for the antioxidant protein in the various tissues examined. In response to the 2,2'-azobiz(2-amidinopropane) dihydrochloride (AAPH) injection, the Tg rats showed a lower level of antioxidant and H2O2 concentration as the activity of the antioxidant enzyme was maintained at a higher level in the Tg rats than in the non-Tg rats. Also, the neutrophil-to-lymphocyte ratio was significantly increased in this Tg rat, even though the level of corticosterone remained unchanged in both genotypes. Thus, the results of this study demonstrated that the CMV/GFP-hSelM Tg rat can serve as an animal model for the maintenance of a high level of antioxidant status and can be used to study the biological function of selenoprotein in infectious diseases, cardiovascular disease and cancer.

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February 2008
Volume 21 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hwang DY, Sin JS, Kim MS, Yim SY, Kim YK, Kim CK, Kim BG, Shim SB, Jee SW, Lee SH, Lee SH, et al: Overexpression of human selenoprotein M differentially regulates the concentrations of antioxidants and H2O2, the activity of antioxidant enzymes, and the composition of white blood cells in a transgenic rat. Int J Mol Med 21: 169-179, 2008.
APA
Hwang, D.Y., Sin, J.S., Kim, M.S., Yim, S.Y., Kim, Y.K., Kim, C.K. ... Chae, K.R. (2008). Overexpression of human selenoprotein M differentially regulates the concentrations of antioxidants and H2O2, the activity of antioxidant enzymes, and the composition of white blood cells in a transgenic rat. International Journal of Molecular Medicine, 21, 169-179. https://doi.org/10.3892/ijmm.21.2.169
MLA
Hwang, D. Y., Sin, J. S., Kim, M. S., Yim, S. Y., Kim, Y. K., Kim, C. K., Kim, B. G., Shim, S. B., Jee, S. W., Lee, S. H., Bae, C. J., Lee, B. C., Jang, M. K., Cho, J. S., Chae, K. R."Overexpression of human selenoprotein M differentially regulates the concentrations of antioxidants and H2O2, the activity of antioxidant enzymes, and the composition of white blood cells in a transgenic rat". International Journal of Molecular Medicine 21.2 (2008): 169-179.
Chicago
Hwang, D. Y., Sin, J. S., Kim, M. S., Yim, S. Y., Kim, Y. K., Kim, C. K., Kim, B. G., Shim, S. B., Jee, S. W., Lee, S. H., Bae, C. J., Lee, B. C., Jang, M. K., Cho, J. S., Chae, K. R."Overexpression of human selenoprotein M differentially regulates the concentrations of antioxidants and H2O2, the activity of antioxidant enzymes, and the composition of white blood cells in a transgenic rat". International Journal of Molecular Medicine 21, no. 2 (2008): 169-179. https://doi.org/10.3892/ijmm.21.2.169