Open Access

Essential nutrients suppress inflammation by modulating key inflammatory gene expression

  • Authors:
    • V. Ivanov
    • J. Cha
    • S. Ivanova
    • T. Kalinovsky
    • M. W. Roomi
    • M. Rath
    • A. Niedzwiecki
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/ijmm_00000079
  • Pages: 731-741
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Abstract

We investigated the effects of a nutrient mixture (NM) consisting of ascorbic acid, quercetin, naringenin, hesperetin, tea catechins, lysine, proline, arginine and N-acetylcysteine on experimental in vivo and in vitro inflammation triggered by bacterial lipopolysaccharide (LPS). BALB/c mice (n=36) were administered NM (200 mg/kg BW) or ibuprofen (20 mg/kg BW) for two weeks. Blood plasma, collected three hours after a single intraperitoneal injection with LPS (1 mg/kg BW), was analyzed with 14 cytokine microarray. LPS inflammatory effects were analyzed in human U937 macrophages by cytokine release, cyclooxygenase (COX) enzymatic activity, COX protein expression (Western blot analysis), specific mRNA levels (RT-PCR), and nuclear factor κβ (NFκβ) activation (phosphorylated p65 immunoassay). Nutrient supplementation in mice altered the LPS-induced cytokine response in a manner similar to ibuprofen (r=0.4157, p=0.139). Cytokine response to LPS in cultured macrophages was similar to the in vivo study (r=0.718, p=0.023). NM inhibited COX-2 enzymatic activity, and COX-2 and pro-inflammatory cytokine protein expression levels were downregulated by NM at the transcription level complementing a blockade in NFκβ activation. NM demonstrated strong beneficial effects on the experimental inflammation by targeting multiple responsible mechanisms in the complex process involved in the inflammatory reaction to pathogens.

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December 2008
Volume 22 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Ivanov, V., Cha, J., Ivanova, S., Kalinovsky, T., Roomi, M.W., Rath, M., & Niedzwiecki, A. (2008). Essential nutrients suppress inflammation by modulating key inflammatory gene expression. International Journal of Molecular Medicine, 22, 731-741. https://doi.org/10.3892/ijmm_00000079
MLA
Ivanov, V., Cha, J., Ivanova, S., Kalinovsky, T., Roomi, M. W., Rath, M., Niedzwiecki, A."Essential nutrients suppress inflammation by modulating key inflammatory gene expression". International Journal of Molecular Medicine 22.6 (2008): 731-741.
Chicago
Ivanov, V., Cha, J., Ivanova, S., Kalinovsky, T., Roomi, M. W., Rath, M., Niedzwiecki, A."Essential nutrients suppress inflammation by modulating key inflammatory gene expression". International Journal of Molecular Medicine 22, no. 6 (2008): 731-741. https://doi.org/10.3892/ijmm_00000079