In vitro cytotoxicity of (-)-EGCG octaacetate on MDAMB-231 and SKHep-1 human carcinoma cells: A pharmacological consideration on prodrug design

  • Authors:
    • Stanton Hon Lung Kok
    • Raymond Siu Ming Wong
    • Roberto Gambari
    • Filly Cheung
    • Wing Sze Lam
    • Fung Yi Lau
    • Gregory Yin Ming Cheng
    • Chor Hing Cheng
    • Kim Hung Lam
    • Sau Hing Chan
    • Johnny Cheuk On Tang
    • Chung Hin Chui
    • Kwok Ping Ho
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/ijmm_00000093
  • Pages: 841-845
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Esterification of acetate with generic pharmaceutical compound has been commonly employed to produce ester prodrug for improving its potency when compared with the mother compound. Acetate, on the other hand, has been recognized to have inhibitory effect on the respiratory biochemistry. Here we demonstrate that acetate at a concentration of 400 µM exhibited significant growth inhibitory activity on two human cancer cell lines, the MDAMB-231 breast cancer and the SKHep-1 hepatoma cell lines. To establish the ester prodrug with multi-acetate ester conjugates as our experimental model, one molecule of (-)-epigallocatechin gallate was required to conjugate with eight molecules of acetate forming the corresponding (-)-epigallocatechin gallate octaacetate prodrug. Chemical structure of this epigallocatechin gallate octaacetate ester prodrug was confirmed by both 13C and 1H nuclear magnetic resonance spectra and mass spectrometry. Further cytotoxic assay using both MDAMB-231 and SKHep-1 human carcinoma cell lines showed that acetate at a concentration of 400 µM exhibits an additional cytotoxic effect with (-)-epigallocatechin gallate at a concentration of 50 µM, although the additional effect was not as high as (-)-epigallocatechin gallate octaacetate ester prodrug alone at a concentration of 50 µM. Our results thus raise a pharmacological consideration of using multi-acetate conjugate as the ester prodrug where the release of free acetate by esterase could be part of the explanation for the improved in vitro cytotoxicity.

Related Articles

Journal Cover

December 2008
Volume 22 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kok SH, Wong RS, Gambari R, Cheung F, Lam WS, Lau FY, Cheng GY, Cheng CH, Lam KH, Chan SH, Chan SH, et al: In vitro cytotoxicity of (-)-EGCG octaacetate on MDAMB-231 and SKHep-1 human carcinoma cells: A pharmacological consideration on prodrug design. Int J Mol Med 22: 841-845, 2008
APA
Kok, S.H., Wong, R.S., Gambari, R., Cheung, F., Lam, W.S., Lau, F.Y. ... Ho, K.P. (2008). In vitro cytotoxicity of (-)-EGCG octaacetate on MDAMB-231 and SKHep-1 human carcinoma cells: A pharmacological consideration on prodrug design. International Journal of Molecular Medicine, 22, 841-845. https://doi.org/10.3892/ijmm_00000093
MLA
Kok, S. H., Wong, R. S., Gambari, R., Cheung, F., Lam, W. S., Lau, F. Y., Cheng, G. Y., Cheng, C. H., Lam, K. H., Chan, S. H., Tang, J. C., Chui, C. H., Ho, K. P."In vitro cytotoxicity of (-)-EGCG octaacetate on MDAMB-231 and SKHep-1 human carcinoma cells: A pharmacological consideration on prodrug design". International Journal of Molecular Medicine 22.6 (2008): 841-845.
Chicago
Kok, S. H., Wong, R. S., Gambari, R., Cheung, F., Lam, W. S., Lau, F. Y., Cheng, G. Y., Cheng, C. H., Lam, K. H., Chan, S. H., Tang, J. C., Chui, C. H., Ho, K. P."In vitro cytotoxicity of (-)-EGCG octaacetate on MDAMB-231 and SKHep-1 human carcinoma cells: A pharmacological consideration on prodrug design". International Journal of Molecular Medicine 22, no. 6 (2008): 841-845. https://doi.org/10.3892/ijmm_00000093