Microarray analysis suggests that burn injury results in mitochondial dysfunction in human skeletal muscle

  • Authors:
    • A. Aria Tzika
    • Dionyssios Mintzopoulos
    • Michael Mindrinos
    • Jiangwen Zhang
    • Laurence G. Rahme
    • Ronald G. Tompkins
  • View Affiliations

  • Published online on: September 1, 2009     https://doi.org/10.3892/ijmm_00000244
  • Pages: 387-392
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Abstract

Burn injuries to extensive areas of the body are complicated by muscle catabolism. Elucidating the molecular mechanisms that mediate this catabolism may facilitate the development of a medical intervention. Here, we assessed the functional classification of genes that were differentially expressed in skeletal muscle following burn injury in 19 children (5.2±4.0 years of age), (64±15% total burn surface area, TBSA) relative to 13 healthy controls (11.9±6.0 years of age). Microarray analysis of samples taken within 10 days of burn injury revealed altered expression of a variety of genes, including some involved in cell and organelle organization and biogenesis, stress response, wound response, external stimulus response, regulation of apoptosis and intracellular signaling. The genes that encode peroxisome proliferator-activated receptors (PPARs; 3 isotypes PPARα, PPARγ and PPARδ also known as PPARβ or PPARβ/δ), which may serve as transcriptional nodal points and therapeutic targets for metabolic syndromes, were among those affected. In particular, expression of the main mitochondrial biogenesis factor PPARγ-1β (or PGC-1β) was downregulated (P<0.0001), while the expression of PPARδ was upregulated (P<0.001). Expression of PGC-1α, the closest homolog of PGC-1β was upregulated (P=0.0037), and expression of the gene encoding mitochodrial uncoupling protein 2 (UCP2) was also upregulated (P=0.008). These results suggest that altered PPAR and mitochondrial gene expression soon after burn injury may lead to metabolic and mitochondrial dysfunction in human skeletal muscle.

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September 2009
Volume 24 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tzika AA, Mintzopoulos D, Mindrinos M, Zhang J, Rahme LG and Tompkins RG: Microarray analysis suggests that burn injury results in mitochondial dysfunction in human skeletal muscle. Int J Mol Med 24: 387-392, 2009.
APA
Tzika, A.A., Mintzopoulos, D., Mindrinos, M., Zhang, J., Rahme, L.G., & Tompkins, R.G. (2009). Microarray analysis suggests that burn injury results in mitochondial dysfunction in human skeletal muscle. International Journal of Molecular Medicine, 24, 387-392. https://doi.org/10.3892/ijmm_00000244
MLA
Tzika, A. A., Mintzopoulos, D., Mindrinos, M., Zhang, J., Rahme, L. G., Tompkins, R. G."Microarray analysis suggests that burn injury results in mitochondial dysfunction in human skeletal muscle". International Journal of Molecular Medicine 24.3 (2009): 387-392.
Chicago
Tzika, A. A., Mintzopoulos, D., Mindrinos, M., Zhang, J., Rahme, L. G., Tompkins, R. G."Microarray analysis suggests that burn injury results in mitochondial dysfunction in human skeletal muscle". International Journal of Molecular Medicine 24, no. 3 (2009): 387-392. https://doi.org/10.3892/ijmm_00000244