Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes

  • Authors:
    • Hong Zhou
    • Haibo Wang
    • Na Li
    • Ying Yu
    • Hongliang Huang
    • Yihong Yan
    • Ting Wang
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijmm_00000265
  • Pages: 557-562
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Abstract

Growing evidence suggests that autoantibodies directly contribute to hypercoagulability in the antiphospholipid syndrome (APS). One proposed mechanism is the antibody-induced expression of tissue factor (TF) by blood monocytes. Annexin A2 (ANX2), a mediator of cell surface-specific plasmin generation, was identified to mediate endothelial cell activation by anti-β2-glycoprotein I (anti-β2GPI) antibody. Our previous study suggested that ANX2 was also involved in anti-β2GPI/β2GPI-induced TF expression on monocytes. In the current study, it was further demonstrated that β2GPI interacts with ANX2 not only in a cell-dependent form but also in a cell-free system. To further confirm the effects of ANX2 on anti-β2GPI/β2GPI-induced TF expression, an ANX2 cDNA-containing vector was transfected into HEK 293T cells which had originally little ANX2, then cells were treated by anti-β2GPI/β2GPI complex. It was found that transfected HEK 293T cells could express more TF both at mRNA and protein levels than that of no-transfected cells. On the other hand, the TF expression was dramatically decreased in the THP-1 cells in which the ANX2 RNA interference was performed. In conclusion, these results indicate that ANX2 on cell surface functions as a mediator boosting TF expression on monocytes induced by anti-β2GPI/β2GPI complex, which is contributed to the thrombotic events in APS.

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October 2009
Volume 24 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhou H, Wang H, Li N, Yu Y, Huang H, Yan Y and Wang T: Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes. Int J Mol Med 24: 557-562, 2009
APA
Zhou, H., Wang, H., Li, N., Yu, Y., Huang, H., Yan, Y., & Wang, T. (2009). Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes. International Journal of Molecular Medicine, 24, 557-562. https://doi.org/10.3892/ijmm_00000265
MLA
Zhou, H., Wang, H., Li, N., Yu, Y., Huang, H., Yan, Y., Wang, T."Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes". International Journal of Molecular Medicine 24.4 (2009): 557-562.
Chicago
Zhou, H., Wang, H., Li, N., Yu, Y., Huang, H., Yan, Y., Wang, T."Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes". International Journal of Molecular Medicine 24, no. 4 (2009): 557-562. https://doi.org/10.3892/ijmm_00000265