The role of VIT1/FBXO11 in the regulation of apoptosis and tyrosinase export from endoplasmic reticulum in cultured melanocytes

Corrigendum in: /10.3892/ijmm.2021.5069

  • Authors:
    • Cuiping Guan
    • Fuquan Lin
    • Miaoni Zhou
    • Weisong Hong
    • Lifang Fu
    • Wen Xu
    • Dongyin Liu
    • Yinsheng Wan
    • Aie Xu
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ijmm_00000435
  • Pages: 57-65
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Our previous study has shown that VIT1 gene in Chinese vitiligo patients is de facto the FBXO11 gene, and the silencing of that gene has an impact on the ultrastructure of melanocytes. In this study, we further identified the role of the FBXO11 gene in melanocytes and the relationship between dilated endoplasmic reticulum (ER) and tyrosinase by inhibition and overexpression of FBXO11 gene. Cell proliferation, apoptosis, cycle and migration of melanocytes were examined when the FBXO11 gene was silenced or overexpressed. The results showed that FBXO11 gene promoted cell proliferation and suppressed cell apoptosis, and yet had little effect on cell migration. Obvious swelling of ER was found in the cells transfected with siRNA of FBXO11 gene. Interestingly, protein level of tyrosinase was extraordinarily high following inhibition of FBXO11 gene. Further examination revealed that tyrosinase and calreticulin were co-localized in ER of transfected cells following siRNA of FBXO11 gene, suggesting that tyrosinase could not be exported from ER effectively. Collectively, our results support the notion that FBXO11 plays an important role in regulating proliferation and apoptosis of melanocytes, and functional export of tyrosinase from ER in vitiligo melanocytes.

Related Articles

Journal Cover

July 2010
Volume 26 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Guan C, Lin F, Zhou M, Hong W, Fu L, Xu W, Liu D, Wan Y and Xu A: The role of VIT1/FBXO11 in the regulation of apoptosis and tyrosinase export from endoplasmic reticulum in cultured melanocytes Corrigendum in /10.3892/ijmm.2021.5069. Int J Mol Med 26: 57-65, 2010.
APA
Guan, C., Lin, F., Zhou, M., Hong, W., Fu, L., Xu, W. ... Xu, A. (2010). The role of VIT1/FBXO11 in the regulation of apoptosis and tyrosinase export from endoplasmic reticulum in cultured melanocytes Corrigendum in /10.3892/ijmm.2021.5069. International Journal of Molecular Medicine, 26, 57-65. https://doi.org/10.3892/ijmm_00000435
MLA
Guan, C., Lin, F., Zhou, M., Hong, W., Fu, L., Xu, W., Liu, D., Wan, Y., Xu, A."The role of VIT1/FBXO11 in the regulation of apoptosis and tyrosinase export from endoplasmic reticulum in cultured melanocytes Corrigendum in /10.3892/ijmm.2021.5069". International Journal of Molecular Medicine 26.1 (2010): 57-65.
Chicago
Guan, C., Lin, F., Zhou, M., Hong, W., Fu, L., Xu, W., Liu, D., Wan, Y., Xu, A."The role of VIT1/FBXO11 in the regulation of apoptosis and tyrosinase export from endoplasmic reticulum in cultured melanocytes Corrigendum in /10.3892/ijmm.2021.5069". International Journal of Molecular Medicine 26, no. 1 (2010): 57-65. https://doi.org/10.3892/ijmm_00000435