Identification of the novel protein FAM172A, and its up-regulation by high glucose in human aortic smooth muscle cells

  • Authors:
    • Lianxi Li
    • Xuehong Dong
    • Man Cheong Leong
    • Wenbai Zhou
    • Zhihong Yang
    • Fengling Chen
    • Yuqian Bao
    • Weiping Jia
    • Renming Hu
  • View Affiliations

  • Published online on: October 1, 2010     https://doi.org/10.3892/ijmm_00000489
  • Pages: 483-490
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Abstract

The family with sequence similarity 172, member A (FAM172A) is a hypothetical protein. We recently cloned the FAM172A gene from normal human aortic tissues. In a previous study we also showed that the FAM172A gene was up-regulated by high glucose levels in macrophages. In the present study, we further identified the FAM172A protein at the level of translation and studied the effects of high glucose levels on its expression in human aortic smooth muscle cells. The FAM172A gene was subcloned into the eukaryotic expression vectors, PDC315 and pEGFP-N2. The cloned sequence shows an open reading frame of 1251 nucleotides encoding a protein of 416 amino acids. We further expressed the recombinant FAM172A protein and generated rabbit anti-human FAM172A polyclonal antibodies. The FAM172A protein was identified for the first time at the translation level by Western blot analysis. Western blotting also demonstrated that the FAM172A protein could be detected in human aortic endothelial, human aortic smooth muscle cells and THP-1-derived macrophages, the highest expression being observed in the human aortic smooth muscle cells. By a combination of bioinformatics and confocal laser scanning microscopy, we found that the FAM172A protein in HEK293 cells, was mainly located in the nucleus, and that there was an Arb2 conserved domain in the FAM172A protein sequence. We also presented evidence that the FAM172 protein expression in human aortic smooth muscle cells was up-regulated by high glucose levels in a concentration-dependent and time-course manner. We speculated that as a novel protein, FAM172A could be involved in the pathogenesis of high glucose-induced vascular damage.

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October 2010
Volume 26 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li L, Dong X, Leong MC, Zhou W, Yang Z, Chen F, Bao Y, Jia W and Hu R: Identification of the novel protein FAM172A, and its up-regulation by high glucose in human aortic smooth muscle cells . Int J Mol Med 26: 483-490, 2010
APA
Li, L., Dong, X., Leong, M.C., Zhou, W., Yang, Z., Chen, F. ... Hu, R. (2010). Identification of the novel protein FAM172A, and its up-regulation by high glucose in human aortic smooth muscle cells . International Journal of Molecular Medicine, 26, 483-490. https://doi.org/10.3892/ijmm_00000489
MLA
Li, L., Dong, X., Leong, M. C., Zhou, W., Yang, Z., Chen, F., Bao, Y., Jia, W., Hu, R."Identification of the novel protein FAM172A, and its up-regulation by high glucose in human aortic smooth muscle cells ". International Journal of Molecular Medicine 26.4 (2010): 483-490.
Chicago
Li, L., Dong, X., Leong, M. C., Zhou, W., Yang, Z., Chen, F., Bao, Y., Jia, W., Hu, R."Identification of the novel protein FAM172A, and its up-regulation by high glucose in human aortic smooth muscle cells ". International Journal of Molecular Medicine 26, no. 4 (2010): 483-490. https://doi.org/10.3892/ijmm_00000489