Frequency and diversity of human immunodeficiency virus type 1 mutations associated with antiretroviral resistance among patients from Southern Brazil failing highly active antiretroviral therapy (HAART)

  • Authors:
    • Flavia Figueiredo Saad
    • Helena Kaminami Morimoto
    • Susana Lilian Wiechmann
    • Ana Maria Bonametti
    • Luis Toshio Ueda
    • Tiemi Matsuo
    • Edna Maria Vissoci Reiche
  • View Affiliations

  • Published online on: October 1, 2010     https://doi.org/10.3892/ijmm_00000503
  • Pages: 585-593
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Abstract

The human immunodeficiency virus type 1 (HIV-1) epidemic in Brazil is spreading to small municipalities as well as the innermost parts of the country and scarce information has been reported on the frequency of HIV-1 resistance-associated mutations in these areas. To determine the frequency and diversity of the HIV-1 antiretroviral resistance-associated mutations among patients failing highly active antiretroviral therapy from Londrina in Southern Brazil, 127 HIV-1 genotyping tests that were assayed during January 2000 to July 2008 from 108 patients were evaluated. Sixty-nine patients (63.9%) were male and 39 (36.1%) were female and the age ranged from 10 to 68 years (mean, 40.8±9.2). All of them showed at least one HIV-1 antiretroviral resistance-associated mutation and in 72 (56.7%) genotyping tests, mutations for the three antiretroviral classes were detected simultaneously. Mutations associated with resistance to protease inhibitor (PI) were detected in 124 tests (97.6%), the main ones were L90M in 28 (22.0%), V82A in 27 (21.2%), M46I in 26 (20.5%), and I54V in 23 (18.1%). The main mutations associated with nucleoside reverse transcriptase inhibitor (NRTI) resistance were M184V in 82 (64.6%), and the thymidine analog mutations were D67N in 51 (40.1%) tests, K70R in 45 (35.4%), T215Y in 40 (31.5%), and M41L in 38 (30.0%). The most frequent major mutations associated with resistance to non-nucleoside RT inhibitors (NNRTI) were K103N in 47 (37.0%), G190A in 11 (8.7%), and G190S in 2 (2.6%) tests. Mutations associated with reduced susceptibility to NRTI and IP simultaneously were observed in 46 (36.2%) tests. The results obtained may contribute to the improvement of the treatment strategies and the management of the antiretroviral drug therapy of HIV-1-infected patients from this Brazilian region, reducing public costs for antiretroviral drugs which have not been efficient in therapy.

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October 2010
Volume 26 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Figueiredo Saad F, Kaminami Morimoto H, Wiechmann SL, Bonametti AM, Ueda LT, Matsuo T and Vissoci Reiche EM: Frequency and diversity of human immunodeficiency virus type 1 mutations associated with antiretroviral resistance among patients from Southern Brazil failing highly active antiretroviral therapy (HAART) . Int J Mol Med 26: 585-593, 2010
APA
Figueiredo Saad, F., Kaminami Morimoto, H., Wiechmann, S.L., Bonametti, A.M., Ueda, L.T., Matsuo, T., & Vissoci Reiche, E.M. (2010). Frequency and diversity of human immunodeficiency virus type 1 mutations associated with antiretroviral resistance among patients from Southern Brazil failing highly active antiretroviral therapy (HAART) . International Journal of Molecular Medicine, 26, 585-593. https://doi.org/10.3892/ijmm_00000503
MLA
Figueiredo Saad, F., Kaminami Morimoto, H., Wiechmann, S. L., Bonametti, A. M., Ueda, L. T., Matsuo, T., Vissoci Reiche, E. M."Frequency and diversity of human immunodeficiency virus type 1 mutations associated with antiretroviral resistance among patients from Southern Brazil failing highly active antiretroviral therapy (HAART) ". International Journal of Molecular Medicine 26.4 (2010): 585-593.
Chicago
Figueiredo Saad, F., Kaminami Morimoto, H., Wiechmann, S. L., Bonametti, A. M., Ueda, L. T., Matsuo, T., Vissoci Reiche, E. M."Frequency and diversity of human immunodeficiency virus type 1 mutations associated with antiretroviral resistance among patients from Southern Brazil failing highly active antiretroviral therapy (HAART) ". International Journal of Molecular Medicine 26, no. 4 (2010): 585-593. https://doi.org/10.3892/ijmm_00000503