Enhanced enteric invasion of scrapie agents into the villous columnar epithelium via maternal immunoglobulin

  • Authors:
    • Yasuhisa Ano
    • Akikazu Sakudo
    • Ryuta Uraki
    • Yukita Sato
    • Jyuri Kono
    • Katsuaki Sugiura
    • Takashi Yokoyama
    • Shigeyoshi Itohara
    • Hiroyuki Nakayama
    • Masayoshi Yukawa
    • Takashi Onodera
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm_00000533
  • Pages: 845-851
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Transmissible spongiform encephalopathies (TSE) are caused by dietary oral exposure to infectious prion proteins (PrPSc); however, the mechanism behind the uptake of PrPSc in the intestines is poorly understood. In addition, epidemiological studies of BSE showed that most cattle are exposed to the agents in the first 6 months of life, during the suckling and weaning periods. In the present study, to elucidate the enteric invasion mechanism of prions and to investigate the age-dependent transmission mechanism suggested by epidemiological studies, wild-type and SCID mice were orally administered brain homogenate from scrapie (Tsukuba 1)-infected mice during the suckling and weaning stages, before being analyzed histopathologically. PrPSc was found to be incorporated into the villous columnar epithelial cells and was also detected in the villous lacteal of 15-day-old suckling mice. However, no such uptake of PrPSc was observed in the weaned mice at 25-days-old. Four different strains of mice were tested. There was no mouse strain difference in the frequency of PrPSc positive columnar epithelial cells. In addition, the uptake of PrPSc in suckling SCID mice lacking maternal antibodies was significantly lower than that in the wild-type suckling mice, and the uptake of PrPSc was enhanced by dilution with purified IgG. In the present study, it was suggested that the weaning period and maternal immunoglobulin are important risk factors for the oral transmission of PrPSc.

Related Articles

Journal Cover

December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ano Y, Sakudo A, Uraki R, Sato Y, Kono J, Sugiura K, Yokoyama T, Itohara S, Nakayama H, Yukawa M, Yukawa M, et al: Enhanced enteric invasion of scrapie agents into the villous columnar epithelium via maternal immunoglobulin . Int J Mol Med 26: 845-851, 2010
APA
Ano, Y., Sakudo, A., Uraki, R., Sato, Y., Kono, J., Sugiura, K. ... Onodera, T. (2010). Enhanced enteric invasion of scrapie agents into the villous columnar epithelium via maternal immunoglobulin . International Journal of Molecular Medicine, 26, 845-851. https://doi.org/10.3892/ijmm_00000533
MLA
Ano, Y., Sakudo, A., Uraki, R., Sato, Y., Kono, J., Sugiura, K., Yokoyama, T., Itohara, S., Nakayama, H., Yukawa, M., Onodera, T."Enhanced enteric invasion of scrapie agents into the villous columnar epithelium via maternal immunoglobulin ". International Journal of Molecular Medicine 26.6 (2010): 845-851.
Chicago
Ano, Y., Sakudo, A., Uraki, R., Sato, Y., Kono, J., Sugiura, K., Yokoyama, T., Itohara, S., Nakayama, H., Yukawa, M., Onodera, T."Enhanced enteric invasion of scrapie agents into the villous columnar epithelium via maternal immunoglobulin ". International Journal of Molecular Medicine 26, no. 6 (2010): 845-851. https://doi.org/10.3892/ijmm_00000533