Lipopolysaccharide promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells via TLR4

  • Authors:
    • Jong-Hwan Park
    • Seong-Min Kwon
    • Hyo-Eun Yoon
    • Soo-A Kim
    • Sang-Gun Ahn
    • Jung-Hoon Yoon
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm.2010.568
  • Pages: 277-281
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Abstract

Odontoblasts and/or dental pulp cells are responsible for tooth repair and dentin formation. Furthermore, adhesion and migration are critical processes for tissue regeneration. This study was performed to clarify whether lipopolysaccharide (LPS) modulates adhesion and migration of the murine odontoblast-like cell line MDPC-23, and whether Toll-like receptor 4 (TLR4) signaling is engaged in this process. TLR4 expression in MDPC-23 cells was examined by RT-PCR. Adhesion assay was performed using type I collagen-coated plates. Migration ability was determined by a commercial assay kit. Phosphorylation of IκB-α, FAK, AKT, and ERK was examined by Western blot analysis. TLR4 was functionally expressed in MDPC-23 cells. LPS treatment enhanced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Blockade of TLR4 using its antibody restored LPS-induced adhesion and migration of MDPC-23 cells. These findings indicate that LPS, an immune activator from Gram-negative bacteria, can promote the adhesion and migration ability of MDPC-23 cells via TLR4.

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February 2011
Volume 27 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Park J, Kwon S, Yoon H, Kim S, Ahn S and Yoon J: Lipopolysaccharide promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells via TLR4. Int J Mol Med 27: 277-281, 2011
APA
Park, J., Kwon, S., Yoon, H., Kim, S., Ahn, S., & Yoon, J. (2011). Lipopolysaccharide promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells via TLR4. International Journal of Molecular Medicine, 27, 277-281. https://doi.org/10.3892/ijmm.2010.568
MLA
Park, J., Kwon, S., Yoon, H., Kim, S., Ahn, S., Yoon, J."Lipopolysaccharide promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells via TLR4". International Journal of Molecular Medicine 27.2 (2011): 277-281.
Chicago
Park, J., Kwon, S., Yoon, H., Kim, S., Ahn, S., Yoon, J."Lipopolysaccharide promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells via TLR4". International Journal of Molecular Medicine 27, no. 2 (2011): 277-281. https://doi.org/10.3892/ijmm.2010.568