Detection of soluble HFE associated with soluble transferrin receptor in human serum

  • Authors:
    • Kouhei Ohnishi
    • Yoshihiro Torimoto
    • Katsuya Ikuta
    • Hiroki Tanaka
    • Takaaki Hosoki
    • Seiji Tanaka
    • Akiei Hamano
    • Kazuya Sato
    • Mikihiro Fujiya
    • Katsunori Sasaki
    • Yutaka Kohgo
  • View Affiliations

  • Published online on: December 16, 2010     https://doi.org/10.3892/ijmm.2010.584
  • Pages: 435-439
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Abstract

Hereditary hemochromatosis is an autosomal recessive disease, and 80-90% of patients exhibit Cys282Tyr or His63Asp mutations in the HFE gene. HFE, also known as major histocompatibility complex (MHC) class I-like molecule, binds to transferrin receptor 1 (TfR1) and β2-microglobulin at the cell surface, forming a complex. Some MHC class I molecules are known to be soluble, raising the possibility that HFE also has a soluble form. However, it is not known whether soluble HFE (sHFE) is present in human serum, and there has been no report on the possible binding between sHFE and soluble TfR (sTfR), which is the fragment of the extracellular domain of TfR1 released into the blood. In the present study, we purified an sTfR complex from pooled serum collected from healthy volunteers, showing that the main components of the complex are sTfR and transferrin. We also confirmed the existence of sHFE in this complex. This is the first report on the existence of sHFE in human serum.

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March 2011
Volume 27 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ohnishi K, Torimoto Y, Ikuta K, Tanaka H, Hosoki T, Tanaka S, Hamano A, Sato K, Fujiya M, Sasaki K, Sasaki K, et al: Detection of soluble HFE associated with soluble transferrin receptor in human serum. Int J Mol Med 27: 435-439, 2011
APA
Ohnishi, K., Torimoto, Y., Ikuta, K., Tanaka, H., Hosoki, T., Tanaka, S. ... Kohgo, Y. (2011). Detection of soluble HFE associated with soluble transferrin receptor in human serum. International Journal of Molecular Medicine, 27, 435-439. https://doi.org/10.3892/ijmm.2010.584
MLA
Ohnishi, K., Torimoto, Y., Ikuta, K., Tanaka, H., Hosoki, T., Tanaka, S., Hamano, A., Sato, K., Fujiya, M., Sasaki, K., Kohgo, Y."Detection of soluble HFE associated with soluble transferrin receptor in human serum". International Journal of Molecular Medicine 27.3 (2011): 435-439.
Chicago
Ohnishi, K., Torimoto, Y., Ikuta, K., Tanaka, H., Hosoki, T., Tanaka, S., Hamano, A., Sato, K., Fujiya, M., Sasaki, K., Kohgo, Y."Detection of soluble HFE associated with soluble transferrin receptor in human serum". International Journal of Molecular Medicine 27, no. 3 (2011): 435-439. https://doi.org/10.3892/ijmm.2010.584