Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity

  • Authors:
    • Ju Dong Song
    • Sang Kwon Lee
    • Si Eun Park
    • Kang Mi Kim
    • Koanhoi Kim
    • Yeong Min Park
    • Young Chul Park
  • View Affiliations

  • Published online on: June 27, 2011     https://doi.org/10.3892/ijmm.2011.736
  • Pages: 841-845
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Abstract

Cobalt protoporphyrin (CoPP) is a potent and effective metalloporphyrin inducer of heme oxygenase-1 (HO-1) activity in many tissues. Here, we report that CoPP induces differentiation of monocytic THP-1 cells into macrophage-like cells. CoPP induced a marked growth inhibition with a slight reduction in viability, and increased adhesion and spreading of THP-1 cells. However, other protoporphyrins did not. CoPP also resulted in expression of CD11b, MMP9, MSR1, CD14 and ICAM-1, which are differentiation markers for macrophages. Interestingly, we observed a decrease of cytoplasmic redox factor-1 (Ref-1) levels in the process of CoPP-induced differentiation of THP-1 cells. In addition, knockdown of Ref-1 by siRNA enhanced cell adhesion induced by CoPP. Furthermore, an inhibitor of NADPH oxidase, diphenyleneiodonium (DPI), completely abolished CoPP-induced adhesion of Ref-1-deficient cells using an siRNA. A cytosolic factor for NADPH oxidase activity, p47phox, was significantly increased in THP-1 cells by CoPP treatment. Κnockdown of Ref-1 increased CoPP-induced p47phox expression in THP-1 cells. Taken together, these results suggest that CoPP induces differentiation of monocytic THP-1 cells, and that the CoPP-induced differentiation is associated with cytoplasmic Ref-1-related NADPH oxidase activity.

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November 2011
Volume 28 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Song JD, Lee SK, Park SE, Kim KM, Kim K, Park YM and Park YC: Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity. Int J Mol Med 28: 841-845, 2011.
APA
Song, J.D., Lee, S.K., Park, S.E., Kim, K.M., Kim, K., Park, Y.M., & Park, Y.C. (2011). Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity. International Journal of Molecular Medicine, 28, 841-845. https://doi.org/10.3892/ijmm.2011.736
MLA
Song, J. D., Lee, S. K., Park, S. E., Kim, K. M., Kim, K., Park, Y. M., Park, Y. C."Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity". International Journal of Molecular Medicine 28.5 (2011): 841-845.
Chicago
Song, J. D., Lee, S. K., Park, S. E., Kim, K. M., Kim, K., Park, Y. M., Park, Y. C."Cobalt protoporphyrin induces differentiation of monocytic THP-1 cells through regulation of cytoplasmic Ref-1-related NADPH oxidase activity". International Journal of Molecular Medicine 28, no. 5 (2011): 841-845. https://doi.org/10.3892/ijmm.2011.736