Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes

  • Authors:
    • Fuquan Lin
    • Wen Xu
    • Cuiping Guan
    • Miaoni Zhou
    • Weisong Hong
    • Lifang Fu
    • Dongyin Liu
    • Aie Xu
  • View Affiliations

  • Published online on: January 12, 2012     https://doi.org/10.3892/ijmm.2012.886
  • Pages: 593-600
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Abstract

Niacin and its related derivatives have been shown to have effects on cellular activities. However, the molecular mechanism of its reduced immunosuppressive effects and photoprotective effects remains unclear. In this study, we investigated the molecular mechanism of the photoprotective effect of niacin in ultraviolet (UV)-irradiated human skin keratinocytes (HaCaT cells). We found that niacin effectively suppressed the UV-induced cell death and cell apoptosis of HaCaT cells. Existing data have shown that AKT activation is involved in the cell survival process. Yet, the potential mechanism of niacin in protection against UV-induced skin damage has thus far not fully been eluvidated. We observed that niacin pretreatment enhances UV induced activation of AKT (Ser473 phosphorylation) as well as that of the downstream signal mTOR (S6 and 4E-BP1 phosphorylation). The PI3K/AKT inhibitor, LY294002, and the mTOR inhibitor, rapamycin, largely neutralized the protective effects of niacin, suggesting that AKT and downstream signaling mTOR/S6 activation are necessary for the niacin-induced protective effects against UV-induced cell death and cell apoptosis. Collectively, our data suggest that niacin may be utilized to prevent UV-induced skin damage and provide a novel mechanism of its photoprotective effects against the UV radiation of sunlight by modulating both AKT and downstream mTOR signaling pathways.
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April 2012
Volume 29 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lin F, Xu W, Guan C, Zhou M, Hong W, Fu L, Liu D and Xu A: Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes. Int J Mol Med 29: 593-600, 2012
APA
Lin, F., Xu, W., Guan, C., Zhou, M., Hong, W., Fu, L. ... Xu, A. (2012). Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes. International Journal of Molecular Medicine, 29, 593-600. https://doi.org/10.3892/ijmm.2012.886
MLA
Lin, F., Xu, W., Guan, C., Zhou, M., Hong, W., Fu, L., Liu, D., Xu, A."Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes". International Journal of Molecular Medicine 29.4 (2012): 593-600.
Chicago
Lin, F., Xu, W., Guan, C., Zhou, M., Hong, W., Fu, L., Liu, D., Xu, A."Niacin protects against UVB radiation-induced apoptosis in cultured human skin keratinocytes". International Journal of Molecular Medicine 29, no. 4 (2012): 593-600. https://doi.org/10.3892/ijmm.2012.886