Detection and identification of peroxiredoxin 3 as a biomarker in hepatocellular carcinoma by a proteomic approach

  • Authors:
    • Bingbing Qiao
    • Jiajia Wang
    • Jianfei Xie
    • Ying Niu
    • Shaojun Ye
    • Qiquan Wan
    • Qifa Ye
  • View Affiliations

  • Published online on: February 15, 2012     https://doi.org/10.3892/ijmm.2012.916
  • Pages: 832-840
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Abstract

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with poor prognosis due to resistance to conventional chemotherapy and limited efficacy of radiotherapy. Thus, alternative therapeutic strategies need to be established. In order to search for a useful biomarker to improve its efficacy, we conducted a two-dimensional gel electrophoresis and MALDI-TOF MS-based comparative proteomic analysis to profile the differentially expressed proteins between HCC tumor tissues with histological evidence and the adjacent non-tumor tissues. Twenty-two out of 43 dysregulated proteins were identified, including 15 upregulated proteins, and 7 downregulated proteins (over 2-fold, P<0.01). The expression of peroxiredoxin 3 (PRDX3) at the mRNA and protein levels was confirmed by RT-PCR and western blotting in HCC cell lines, and HCC samples, and further analysed by immunohistochemistry in HCC samples of different clinical pathological stages. The results indicated that overexpression of PRDX3 was associated with 94.9% HCC, and correlated with poor differentiation (P<0.05), which suggest that PRDX3 has substantial clinical impact on the progression of hepatocarcinoma, and may be a potential therapeutic target for HCC.

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May 2012
Volume 29 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Qiao B, Wang J, Xie J, Niu Y, Ye S, Wan Q and Ye Q: Detection and identification of peroxiredoxin 3 as a biomarker in hepatocellular carcinoma by a proteomic approach. Int J Mol Med 29: 832-840, 2012.
APA
Qiao, B., Wang, J., Xie, J., Niu, Y., Ye, S., Wan, Q., & Ye, Q. (2012). Detection and identification of peroxiredoxin 3 as a biomarker in hepatocellular carcinoma by a proteomic approach. International Journal of Molecular Medicine, 29, 832-840. https://doi.org/10.3892/ijmm.2012.916
MLA
Qiao, B., Wang, J., Xie, J., Niu, Y., Ye, S., Wan, Q., Ye, Q."Detection and identification of peroxiredoxin 3 as a biomarker in hepatocellular carcinoma by a proteomic approach". International Journal of Molecular Medicine 29.5 (2012): 832-840.
Chicago
Qiao, B., Wang, J., Xie, J., Niu, Y., Ye, S., Wan, Q., Ye, Q."Detection and identification of peroxiredoxin 3 as a biomarker in hepatocellular carcinoma by a proteomic approach". International Journal of Molecular Medicine 29, no. 5 (2012): 832-840. https://doi.org/10.3892/ijmm.2012.916