Immune activation and antitumor response of ar-turmerone on P388D1 lymphoblast cell implanted tumors

  • Authors:
    • Donghee Kim
    • Yongjoon Suh
    • Hyunsook Lee
    • Yongkyu Lee
  • View Affiliations

  • Published online on: November 29, 2012     https://doi.org/10.3892/ijmm.2012.1196
  • Pages: 386-392
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Abstract

Aromatic turmerone (ar-turmerone) has been reported to have a cytotoxic effect on L-1210 and HL-60 cells. In the present study, we investigated the anticancer responses and immune activities in implanted tumor cells. Our study found that ar-turmerone inhibited the increase in the number of white blood cells, which normally increase by the injection of lymphoblast cells, or P388D1, and ar-turmerone increased lymphocyte percentage compared to the control. Tumor inhibition rate in the ar-turmerone-treated group was 11.79%, and the apoptosis indexes of the control, ar-turmerone and Glivec groups were 4.22±1.02, 5.45±1.46 and 10.01±2.01, respectively, in which only the Glivec-treated group showed a significance. The positive rates of Bcl-2 and Bax proteins which were treated by ar-turmerone did not show marked differences compared to the control group, but the Bax protein in the Glivec-treated group increased compared to the control group. The density of caspase-1, -3, -6, -9, Bcl-2, Bax, p21 and p53 mRNA in the control, ar-turmerone and Glivec groups did not change considerably, but the Bax mRNA of the Glivec-treated group increased compared to the control group. The ar-turmerone-treated group increased T-lymphocyte and B-lymphocyte proliferation activities compared to the control group, which was more significant in T-lymphocyte than in B-lymphocyte proliferation activity. The interleukin-2 (IL2) production activity of the ar-turmerone group increased compared to the control group. These findings suggest that ar-turmerone does not have a chemotherapeutic effect on tumor incidence, but it has a repressive effect on P388D1 lymphocytic leukemia. Furthermore, this protective effect of ar-turmerone from P388D1 lymphocytic leukemia resulted from the increased activity of tumor immunogenicity through increased T-lymphokine production and increased percentage of lymphocytes.

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February 2013
Volume 31 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Kim, D., Suh, Y., Lee, H., & Lee, Y. (2013). Immune activation and antitumor response of ar-turmerone on P388D1 lymphoblast cell implanted tumors. International Journal of Molecular Medicine, 31, 386-392. https://doi.org/10.3892/ijmm.2012.1196
MLA
Kim, D., Suh, Y., Lee, H., Lee, Y." Immune activation and antitumor response of ar-turmerone on P388D1 lymphoblast cell implanted tumors". International Journal of Molecular Medicine 31.2 (2013): 386-392.
Chicago
Kim, D., Suh, Y., Lee, H., Lee, Y." Immune activation and antitumor response of ar-turmerone on P388D1 lymphoblast cell implanted tumors". International Journal of Molecular Medicine 31, no. 2 (2013): 386-392. https://doi.org/10.3892/ijmm.2012.1196