Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB

Hwang,Bo-Mi; Noh,Eun-Mi; Kim,Jong-Suk; Kim,Jeong-Mi; Hwang,Jin-Ki; Kim,Hye-Kyung; Kang,Jae-Seon; Kim,Do-Sung; Chae,Han-Jung; You,Yong-Ouk; Kwon,Kang-Beom; Lee,Young-Rae

doi:10.3892/ijmm.2012.1202

Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB

Authors:
- Bo-Mi Hwang
- Eun-Mi Noh
- Jong-Suk Kim
- Jeong-Mi Kim
- Jin-Ki Hwang
- Hye-Kyung Kim
- Jae-Seon Kang
- Do-Sung Kim
- Han-Jung Chae
- Yong-Ouk You
- Kang-Beom Kwon
- Young-Rae Lee
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Affiliations: Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea, Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea, Department of Urology, Medical School, and Institute for Medical Sciences, Chonbuk National University, Jeonju 561-712, Republic of Korea, Department of Pharmacy, Kyungsung University, Busan 608-736, Republic of Korea, Department of Pharmacology, School of Medicine, Chonbuk National University, Jeonju 560-182, Republic of Korea, Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea
Published online on: December 4, 2012 https://doi.org/10.3892/ijmm.2012.1202
Pages: 477-483

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Abstract

Decursin, a coumarin compound, was originally isolated from the roots of Angelica gigas almost four decades ago, and it was found to exhibit cytotoxicity against various types of human cancer cells and anti-amnesic activity in vivo through the inhibition of AChE activity. However, the anti-skin photoaging effects of decursin have not been reported to date. In the present study, we investigated the inhibitory effects of decursin on the expression of matrix metalloproteinase (MMP)-1 and MMP-3 in human dermal fibroblast (HDF) cells. Western blot analysis and real-time PCR revealed that decursin inhibited the ultraviolet (UV)B-induced expression of MMP-1 and MMP-3 in a dose-dependent manner. Decursin significantly blocked the UVB-induced activation of nuclear factor-κB (NF-κB). However, decursin showed no effect on MAPK or AP-1 activity. In this study, decursin prevented the UVB-induced expression of MMPs via the inhibition of NF-κB activation. In conclusion, decursin may be a potential agent for the prevention and treatment of skin photoaging.

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