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Article

Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways

  • Authors:
    • Yali Chen
    • Jinyao Jiang
    • Huibing Miao
    • Xingjuan Chen
    • Xuejun Sun
    • Yongjun Li
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China, Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Pharmacology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Diving Medicine, Faculty of Naval Medicine, The Second Military Medical University, Shanghai 200433, P.R. China
  • Pages: 597-606
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    Published online on: January 22, 2013
       https://doi.org/10.3892/ijmm.2013.1256
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Abstract

Hydrogen-rich saline has been reported to prevent neointimal hyperplasia induced by carotid balloon injury. The purpose of the present study was to further investigate the molecular mechanisms underlying this phenomenon. Daily injection of a hydrogen-rich saline solution (HRSS) in rats was employed to study the effect of hydrogen on balloon injury-induced neointimal hyperplasia and the neointima/media ratio was assessed. HRSS significantly decreased the neointima area and neointima/media ratio in a dose-dependent manner. In vitro effects of hydrogen on fetal bovine serum (FBS)-induced vascular smooth muscle cell (VSMC) proliferation were also investigated. Hydrogen-rich medium (HRM) inhibited rat VSMC proliferation and migration induced by 10% FBS. FBS-induced reactive oxygen species (ROS) production and activation of intracellular Ras, MEK1/2, ERK1/2, proliferative cell nuclear antigen (PCNA), Akt were significantly inhibited by HRM. In addition, HRM blocked FBS-induced progression from the G0/G1 to the S-phase and increased the apoptosis rate of VSMCs. These results showed that hydrogen-rich saline was able to attenuate FBS-induced VSMC proliferation and neointimal hyperplasia by inhibiting ROS production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways. Thus, HRSS may have potential therapeutic relevance for the prevention of human restenosis.
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View References

1 

Landau C, Lange RA and Hillis LD: Percutaneous transluminal coronary angioplasty. N Engl J Med. 330:981–993. 1994. View Article : Google Scholar : PubMed/NCBI

2 

van Domburg RT, Foley DP, Breeman A, van Herwerden LA and Serruys PW: Coronary artery bypass graft surgery and percutaneous transluminal coronary angioplasty. Twenty-year clinical outcome. Eur Heart J. 23:543–549. 2002.PubMed/NCBI

3 

Sturek M and Reddy HK: New tools for prevention of restenosis could decrease the ‘oculo-stento’ reflex. Cardiovasc Res. 53:292–293. 2002.PubMed/NCBI

4 

Sasaki T, Maruyama H, Kase Y, Takeda S and Aburada M: Antianginal effects of lercanidipine on the vasopressin or methacholine induced anginal model in rats. Biol Pharm Bull. 28:811–816. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Park J, Ha H, Seo J, et al: Mycophenolic acid inhibits platelet-derived growth factor-induced reactive oxygen species and mitogen-activated protein kinase activation in rat vascular smooth muscle cells. Am J Transplant. 4:1982–1990. 2004. View Article : Google Scholar

6 

Ohsawa I, Ishikawa M, Takahashi K, et al: Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 13:688–694. 2007. View Article : Google Scholar

7 

Hong Y, Chen S and Zhang JM: Hydrogen as a selective antioxidant: a review of clinical and experimental studies. J Int Med Res. 38:1893–1903. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Wang C, Li J, Liu Q, et al: Hydrogen-rich saline reduces oxidative stress and inflammation by inhibit of JNK and NF-kappaB activation in a rat model of amyloid-beta-induced Alzheimer’s disease. Neurosci Lett. 491:127–132. 2011.PubMed/NCBI

9 

Sun H, Chen L, Zhou W, et al: The protective role of hydrogen-rich saline in experimental liver injury in mice. J Hepatol. 54:471–480. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Sun Q, Kang Z, Cai J, et al: Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats. Exp Biol Med. 234:1212–1219. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Kajiya M, Silva MJ, Sato K, Ouhara K and Kawai T: Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochem Biophys Res Commun. 386:11–15. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Nakao A, Kaczorowski DJ, Wang Y, et al: Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both. J Heart Lung Transplant. 29:544–553. 2010. View Article : Google Scholar

13 

Hayashida K, Sano M, Ohsawa I, et al: Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemiareperfusion injury. Biochem Biophys Res Commun. 373:30–35. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Qin ZX, Yu P, Qian DH, et al: Hydrogen-rich saline prevents neointima formation after carotid balloon injury by suppressing ROS and the TNF-alpha/NF-kappaB pathway. Atherosclerosis. 220:343–350. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Wang F, Yu G, Liu SY, et al: Hydrogen-rich saline protects against renal ischemia/reperfusion injury in rats. J Surg Res. 167:e339–e344. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Shingu C, Koga H, Hagiwara S, et al: Hydrogen-rich saline solution attenuates renal ischemia-reperfusion injury. J Anesth. 24:569–574. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Sun Q, Cai J, Zhou J, et al: Hydrogen-rich saline reduces delayed neurologic sequelae in experimental carbon monoxide toxicity. Crit Care Med. 39:765–769. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Ohsawa I, Nishimaki K, Yamagata K, Ishikawa M and Ohta S: Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice. Biochem Biophys Res Commun. 377:1195–1198. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Itoh T, Fujita Y, Ito M, et al: Molecular hydrogen suppresses FcepsilonRI-mediated signal transduction and prevents degranulation of mast cells. Biochem Biophys Res Commun. 389:651–656. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Zheng X, Mao Y, Cai J, et al: Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats. Free Radic Res. 43:478–484. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Clowes AW, Reidy MA and Clowes MM: Kinetics of cellular proliferation after arterial injury. I Smooth muscle growth in the absence of endothelium. Lab Invest. 49:327–333. 1983.PubMed/NCBI

22 

Wei GL, Krasinski K, Kearney M, Isner JM, Walsh K and Andres V: Temporally and spatially coordinated expression of cell cycle regulatory factors after angioplasty. Circ Res. 80:418–426. 1997.PubMed/NCBI

23 

Yeh JL, Liou SF, Chang YP, et al: Isoeugenodilol inhibits smooth muscle cell proliferation and neointimal thickening after balloon injury via inactivation of ERK1/2 pathway. J Biomed Sci. 15:375–389. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Liou SF, Yeh JL, Liang JC, Chiu CC, Lin YT and Chen IJ: Inhibition of mitogen-mediated proliferation of rat vascular smooth muscle cells by labedipinedilol-A through PKC and ERK 1/2 pathway. J Cardiovasc Pharmacol. 44:539–551. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Parenti A, Bellik L, Brogelli L, Filippi S and Ledda F: Endogenous VEGF-A is responsible for mitogenic effects of MCP-1 on vascular smooth muscle cells. Am J Physiol Heart Circ Physiol. 286:H1978–H1984. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Berridge MV, Herst PM and Tan AS: Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 11:127–152. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Muir D, Varon S and Manthorpe M: An enzyme-linked immunosorbent assay for bromodeoxyuridine incorporation using fixed microcultures. Anal Biochem. 185:377–382. 1990. View Article : Google Scholar : PubMed/NCBI

28 

Li JJ, Han M, Wen JK and Li AY: Osteopontin stimulates vascular smooth muscle cell migration by inducing FAK phosphorylation and ILK dephosphorylation. Biochem Biophys Res Commun. 356:13–19. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Fishbein MC, Wang T, Matijasevic M, Hong L and Apple FS: Myocardial tissue troponins T and I. An immunohistochemical study in experimental models of myocardial ischemia. Cardiovasc Pathol. 12:65–71. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Salvemini D, Riley DP and Cuzzocrea S: SOD mimetics are coming of age. Nat Rev Drug Discov. 1:367–374. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Schwartz RS and Henry TD: Pathophysiology of coronary artery restenosis. Rev Cardiovasc Med. 3(Suppl 5): S4–S9. 2002.

32 

Braun-Dullaeus RC, Mann MJ and Dzau VJ: Cell cycle progression: new therapeutic target for vascular proliferative disease. Circulation. 98:82–89. 1998. View Article : Google Scholar : PubMed/NCBI

33 

Chen Z, Lee FY, Bhalla KN and Wu J: Potent inhibition of platelet-derived growth factor-induced responses in vascular smooth muscle cells by BMS-354825 (dasatinib). Mol Pharmacol. 69:1527–1533. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Stettler C, Wandel S, Allemann S, et al: Outcomes associated with drug-eluting and bare-metal stents: a collaborative network meta-analysis. Lancet. 370:937–948. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Lemos PA, Saia F, Ligthart JM, et al: Coronary restenosis after sirolimus-eluting stent implantation: morphological description and mechanistic analysis from a consecutive series of cases. Circulation. 108:257–260. 2003. View Article : Google Scholar

36 

Fujii K, Mintz GS, Kobayashi Y, et al: Contribution of stent underexpansion to recurrence after sirolimus-eluting stent implantation for in-stent restenosis. Circulation. 109:1085–1088. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Takebayashi H, Kobayashi Y, Mintz GS, et al: Intravascular ultrasound assessment of lesions with target vessel failure after sirolimus-eluting stent implantation. Am J Cardiol. 95:498–502. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Griendling KK and Ushio-Fukai M: Redox control of vascular smooth muscle proliferation. J Lab Clin Med. 132:9–15. 1998. View Article : Google Scholar : PubMed/NCBI

39 

Kunsch C and Medford RM: Oxidative stress as a regulator of gene expression in the vasculature. Circ Res. 85:753–766. 1999. View Article : Google Scholar : PubMed/NCBI

40 

Boullier A, Bird DA, Chang MK, et al: Scavenger receptors, oxidized LDL, and atherosclerosis. Ann NY Acad Sci. 947:214–223. 2001. View Article : Google Scholar : PubMed/NCBI

41 

Mizuno Y, Jacob RF and Mason RP: Inflammation and the development of atherosclerosis. J Atheroscler Thromb. 18:351–358. 2011. View Article : Google Scholar : PubMed/NCBI

42 

Szocs K, Lassegue B, Sorescu D, et al: Upregulation of Nox-based NAD(P)H oxidases in restenosis after carotid injury. Arterioscler Thromb Vasc Biol. 22:21–27. 2002. View Article : Google Scholar : PubMed/NCBI

43 

Shi Y, Niculescu R, Wang D, Patel S, Davenpeck KL and Zalewski A: Increased NAD(P)H oxidase and reactive oxygen species in coronary arteries after balloon injury. Arterioscler Thromb Vasc Biol. 21:739–745. 2001. View Article : Google Scholar : PubMed/NCBI

44 

Kasai H: Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res. 387:147–163. 1997.

45 

Roberts PJ and Der CJ: Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 26:3291–3310. 2007. View Article : Google Scholar : PubMed/NCBI

46 

Lewis TS, Shapiro PS and Ahn NG: Signal transduction through MAP kinase cascades. Adv Cancer Res. 74:49–139. 1998. View Article : Google Scholar : PubMed/NCBI

47 

Lee JH, Johnson PR, Roth M, Hunt NH and Black JL: ERK activation and mitogenesis in human airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 280:L1019–L1029. 2001.PubMed/NCBI

48 

Koyama H, Olson NE, Dastvan FF and Reidy MA: Cell replication in the arterial wall: activation of signaling pathway following in vivo injury. Circ Res. 82:713–721. 1998. View Article : Google Scholar : PubMed/NCBI

49 

Roovers K, Davey G, Zhu X, Bottazzi ME and Assoian RK: Alpha5beta1 integrin controls cyclin D1 expression by sustaining mitogen-activated protein kinase activity in growth factor-treated cells. Mol Biol Cell. 10:3197–3204. 1999. View Article : Google Scholar : PubMed/NCBI

50 

Hirata A, Igarashi M, Yamaguchi H, et al: Nifedipine suppresses neointimal thickening by its inhibitory effect on vascular smooth muscle cell growth via a MEK-ERK pathway coupling with Pyk2. Br J Pharmacol. 131:1521–1530. 2000. View Article : Google Scholar : PubMed/NCBI

51 

Wu JR, Liou SF, Lin SW, et al: Lercanidipine inhibits vascular smooth muscle cell proliferation and neointimal formation via reducing intracellular reactive oxygen species and inactivating Ras-ERK1/2 signaling. Pharmacol Res. 59:48–56. 2009. View Article : Google Scholar

52 

Lawlor MA and Alessi DR: PKB/Akt: a key mediator of cell proliferation, survival and insulin responses? J Cell Sci. 114:2903–2910. 2001.PubMed/NCBI

53 

Ranganna K, Yatsu FM, Hayes BE, Milton SG and Jayakumar A: Butyrate inhibits proliferation-induced proliferating cell nuclear antigen expression (PCNA) in rat vascular smooth muscle cells. Mol Cell Biochem. 205:149–161. 2000. View Article : Google Scholar

54 

Wu CH, Lin CS, Hung JS, et al: Inhibition of neointimal formation in porcine coronary artery by a Ras mutant. J Surg Res. 99:100–106. 2001. View Article : Google Scholar : PubMed/NCBI

55 

Grube E, Gerckens U, Muller R and Bullesfeld L: Drug eluting stents: initial experiences. Z Kardiol. 91(Suppl 3): S44–S48. 2002. View Article : Google Scholar : PubMed/NCBI

56 

Donovan M and Cotter TG: Control of mitochondrial integrity by Bcl-2 family members and caspase-independent cell death. Biochim Biophys Acta. 1644:133–147. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Jiang J, Miao H, Chen X, Sun X and Li Y: Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways. Int J Mol Med 31: 597-606, 2013.
APA
Chen, Y., Jiang, J., Miao, H., Chen, X., Sun, X., & Li, Y. (2013). Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways. International Journal of Molecular Medicine, 31, 597-606. https://doi.org/10.3892/ijmm.2013.1256
MLA
Chen, Y., Jiang, J., Miao, H., Chen, X., Sun, X., Li, Y."Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways". International Journal of Molecular Medicine 31.3 (2013): 597-606.
Chicago
Chen, Y., Jiang, J., Miao, H., Chen, X., Sun, X., Li, Y."Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways". International Journal of Molecular Medicine 31, no. 3 (2013): 597-606. https://doi.org/10.3892/ijmm.2013.1256
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Jiang J, Miao H, Chen X, Sun X and Li Y: Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways. Int J Mol Med 31: 597-606, 2013.
APA
Chen, Y., Jiang, J., Miao, H., Chen, X., Sun, X., & Li, Y. (2013). Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways. International Journal of Molecular Medicine, 31, 597-606. https://doi.org/10.3892/ijmm.2013.1256
MLA
Chen, Y., Jiang, J., Miao, H., Chen, X., Sun, X., Li, Y."Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways". International Journal of Molecular Medicine 31.3 (2013): 597-606.
Chicago
Chen, Y., Jiang, J., Miao, H., Chen, X., Sun, X., Li, Y."Hydrogen-rich saline attenuates vascular smooth muscle cell proliferation and neointimal hyperplasia by inhibiting reactive oxygen species production and inactivating the Ras-ERK1/2-MEK1/2 and Akt pathways". International Journal of Molecular Medicine 31, no. 3 (2013): 597-606. https://doi.org/10.3892/ijmm.2013.1256
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