Wasf2: A novel target of intermittent parathyroid hormone administration

Uyama,Maki; Kawanami,Masamitsu; Tamura,Masato

doi:10.3892/ijmm.2013.1315

Wasf2: A novel target of intermittent parathyroid hormone administration

Authors:
- Maki Uyama
- Masamitsu Kawanami
- Masato Tamura
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Affiliations: Department of Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan, Department of Periodontology and Endodontology, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan
Published online on: March 22, 2013 https://doi.org/10.3892/ijmm.2013.1315
Pages: 1243-1247

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Abstract

Systemic intermittent administration of parathyroid hormone (PTH) stimulates bone formation in animals and humans, and recombinant human PTH1-34 (teriparatide) is used clinically for the treatment of osteoporosis. In this study, we investigated the regulation of gene expression by intermittent PTH administration in MC3T3-E1 osteoblastic cells. We found that intermittent PTH1-34 administration downregulated Wiskott-Aldrich syndrome protein family member (Wasf) 2 mRNA expression. Wnt inhibitor, IWP-2, and protein kinase C inhibitor, Go6976, inhibited this downregulation. However, continuous PTH did not affect Wasf2 expression. Transfection of Wasf2 siRNA reduced bone sialoprotein (BSP) mRNA expression in a similar manner following intermittent PTH administration in MC3T3-E1 cells. These results identify Wasf2 as a novel target of intermittent PTH administration via the Wnt and phosphoinositide-dependent protein kinase signaling pathways, and the resulting regulation of BSP expression may contribute to the anabolic effects of PTH.

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