Electroacupuncture promotes chondrocyte proliferation via accelerated G1/S transition in the cell cycle

  • Authors:
    • Yali Huang
    • Guangwen Wu
    • Huailing Fan
    • Jinxia Ye
    • Xianxiang Liu
  • View Affiliations

  • Published online on: April 5, 2013     https://doi.org/10.3892/ijmm.2013.1336
  • Pages: 1443-1448
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Abstract

The aim of the present study was to investigate the effects of electroacupuncture (EA) on the proliferation of chondrocytes and the molecular mechanism(s) involved. Passage 2 chondrocytes were randomly divided into four groups and treated with EA or nocodazole. After treatment, cell proliferation was determined using an MTT assay and DNA staining followed by FACS. The mRNA expression levels of cyclin D1, cyclin-dependent kinase (CDK)4, CDK6, phosphorylated retinoblastoma (pRb) and P16 were detected by RT-PCR, and the protein levels of cyclin D1, CDK4, CDK6, pRb and P16 were detected by western blotting. EA treatment significantly increased cell viability in a time-dependent manner and decreased the number of G0/G1 and G2/M phase chondrocytes and increased the number of S phase cells. The mRNA and protein levels of cyclin D1, CDK4, CDK6, (p)Rb and P16 consistently demonstrated a reverse trend with the levels in the chondrocytes treated with nocodazole. The expression levels of cyclin D1, CDK4, CDK6 and Rb were higher in chondrocytes receiving EA treatment when compared to levels in the untreated cells while expression of P16 was lower. In conclusion, EA treatment promotes chondrocyte proliferation via promotion of G1/S checkpoint transition in the cell cycle dependent on the activity of the P16-cyclin D1-CDK4/6-pRb pathway and this may, in part, explain its clinical effect in the treatment of osteoarthritis.
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June 2013
Volume 31 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Huang Y, Wu G, Fan H, Ye J and Liu X: Electroacupuncture promotes chondrocyte proliferation via accelerated G1/S transition in the cell cycle. Int J Mol Med 31: 1443-1448, 2013.
APA
Huang, Y., Wu, G., Fan, H., Ye, J., & Liu, X. (2013). Electroacupuncture promotes chondrocyte proliferation via accelerated G1/S transition in the cell cycle. International Journal of Molecular Medicine, 31, 1443-1448. https://doi.org/10.3892/ijmm.2013.1336
MLA
Huang, Y., Wu, G., Fan, H., Ye, J., Liu, X."Electroacupuncture promotes chondrocyte proliferation via accelerated G1/S transition in the cell cycle". International Journal of Molecular Medicine 31.6 (2013): 1443-1448.
Chicago
Huang, Y., Wu, G., Fan, H., Ye, J., Liu, X."Electroacupuncture promotes chondrocyte proliferation via accelerated G1/S transition in the cell cycle". International Journal of Molecular Medicine 31, no. 6 (2013): 1443-1448. https://doi.org/10.3892/ijmm.2013.1336