Anti-inflammatory effects of tectroside on UVB-induced HaCaT cells

Kim,Sung-Bae; Kang,Ok-Hwa; Joung,Dae-Ki; Mun,Su-Hyun; Seo,Yun-Soo; Cha,Mi-Ran; Ryu,Shi-Yong; Shin,Dong-Won; Kwon,Dong-Yeul

doi:10.3892/ijmm.2013.1343

Anti-inflammatory effects of tectroside on UVB-induced HaCaT cells

Authors:
- Sung-Bae Kim
- Ok-Hwa Kang
- Dae-Ki Joung
- Su-Hyun Mun
- Yun-Soo Seo
- Mi-Ran Cha
- Shi-Yong Ryu
- Dong-Won Shin
- Dong-Yeul Kwon
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Affiliations: College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University, Jeonbuk 570-749, Republic of Korea, Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea, Department of Oriental Medicine Resources, Sunchon National University, Jeonnam 540-742, Republic of Korea
Published online on: April 11, 2013 https://doi.org/10.3892/ijmm.2013.1343
Pages: 1471-1476

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Abstract

Ultraviolet B (UVB) irradiation causes skin damage and inflammation by inducing the secretion of various cytokines, which are immune regulators produced by cells. To prevent skin inflammation, keratinocytes that have been irreversibly damaged by UVB must be eliminated through apoptosis. Ixeris dentata (I. dentata) (family Asteraceae) is a perennial medicinal herb indigenous to Korea. It is used in Korea, China and Japan to treat indigestion, pneumonia, diabetes, hepatitis, contusions and tumors. Guaiane-type sesquiterpene lactones were isolated from the whole extract of I. dentata. This led to the isolation of the anti-inflammatory sesquiterpene lactone compound tectroside (TES), which was tested on a human keratinocyte cell line. To determine the anti-inflammatory effects of TES, we examined its influence on UVB-induced pro-inflammatory cytokine production in human keratinocytes (HaCaT cells) by observing these cells in the presence or absence of TES. In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction and western blot analysis to evaluate the activation of mitogen-activated protein kinases (MAPKs). TES inhibited UVB-induced production of the pro-inflammatory cytokines interleukin (IL)-6 and IL-8 in a dose-dependent manner. In addition, TES inhibited the expression of cyclooxygenase (COX)-2 and the phosphorylation of c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) MAPKs, suggesting that it inhibits the secretion of the pro-inflammatory cytokines IL-6 and IL-8 and COX-2 expression by blocking MAPK phosphorylation. These results suggest that TES can potentially protect against UVB-induced skin inflammation.

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1	Murphy GF: Structure, function and reaction patterns. Dermatopathology. W.B. Saunders; Philadelphia, PA: pp. 251995
2	Yager J: The skin as an immune organ. Advances in Veterinary Dermatology. Ihrke PJ, Mason IS and White SD: Pergamon Press; Oxford: pp. 311993
3	de Gruijl FR, Sterenborg HJ, Forbes PD, Davies RE, Cole C, Kelfkens G, van Weelden H, Slaper H and van der Leun JC: Wavelength dependence of skin cancer induction by ultraviolet irradiation of albino hairless mice. Cancer Res. 53:53–60. 1993.PubMed/NCBI
4	van der Leun J and de Gruijl F: Influence of ozone depletion on human and animal health. UV-B Radiation and Ozone Depletion: Effects on Humans, Animals, Plants, Microorganisms and Materials. Tevini M: Lewis Publishers; Boca Raton, FL: pp. 95–123. 1993
5	Rosette C and Karin M: Ultraviolet light and osmotic stress: activation of the JNK cascade through multiple growth factor and cytokine receptors. Science. 274:1194–1197. 1996. View Article : Google Scholar : PubMed/NCBI
6	Afaq F, Adhami VM and Mukhtar H: Photochemoprevention of ultraviolet B signaling and photocarcinogenesis. Mutat Res. 571:153–173. 2005. View Article : Google Scholar : PubMed/NCBI
7	Yaar M and Gilchrest BA: Photoageing: mechanism, prevention and therapy. Br J Dermatol. 157:874–887. 2007. View Article : Google Scholar : PubMed/NCBI
8	Malorni W, Donelli G, Straface E, Santini MT, Paradisi S and Giacomoni PU: Both UVA and UVB induce cytoskeleton-dependent surface blebbing in epidermoid cells. J Photochem Photobiol B. 26:265–270. 1994. View Article : Google Scholar : PubMed/NCBI
9	Zamansky GB, Perrino BA and Chou IN: Disruption of cytoplasmic microtubules by ultraviolet radiation. Exp Cell Res. 195:269–273. 1991. View Article : Google Scholar : PubMed/NCBI
10	Zamansky GB and Chou IN: Disruption of keratin intermediate filaments by ultraviolet radiation in cultured human keratinocytes. Photochem Photobiol. 52:903–906. 1990.PubMed/NCBI
11	Zamansky GB and Chou IN: Environmental wavelengths of ultraviolet light induce cytoskeletal damage. J Invest Dermatol. 89:603–606. 1987. View Article : Google Scholar : PubMed/NCBI
12	Moll I, Bohnert E, Treib U and Jung EG: Effects of ultraviolet B radiation on cytoskeletal and adhesion molecules in human epidermis. Photodermatol Photoimmunol Photomed. 10:26–32. 1994.PubMed/NCBI
13	Godar DE: Preprogrammed and programmed cell death mechanisms of apoptosis: UV-induced immediate and delayed apoptosis. Photochem Photobiol. 63:825–830. 1996. View Article : Google Scholar : PubMed/NCBI
14	Shindo Y and Hashimoto T: Ultraviolet B-induced cell death in four cutaneous cell lines exhibiting different enzymatic antioxidant defences: involvement of apoptosis. J Dermatol Sci. 17:140–150. 1998. View Article : Google Scholar : PubMed/NCBI
15	Kim SH: Inhibitory effects of Ixeris dentata on the mutagenicity of aflatoxin B1, N-methyl-N’-nitro-N-nitrosoguanidine and the growth of MG-63 human osteosarcoma cells. J Korean Soc Food Sci Nutr. 24:305–312. 1995.
16	Kim M and Lee M: Volatile flavor components of Ixeris dentata and Amaranthus mangostanus. Han’guk Nonghwa Hakhoe chi. 31:394–399. 1988.(In Korean).
17	Lee E: Effects of Ixeris dentata ext. on lowering lipid and antioxidation. Korean J Plant Res. 24:55–60. 2011.(In Korean).
18	Park EK, Sung JH, Trinh HT, Bae EA, Yun HK, Hong SS and Kim DH: Lactic acid bacterial fermentation increases the antiallergic effects of Ixeris dentata. J Microbiol Biotechnol. 18:308–313. 2008.PubMed/NCBI
19	Chung HS: Inhibition of monamine oxidase by a flavone and its glycoside from Ixeris dentata Nakai. Nutraceut Food. 8:141–144. 2003. View Article : Google Scholar
20	Chung HS, Jeong HJ, Han MJ, Park ST, Seong KK, Baek SH, Jeong DM, Kim MJ and Kim HM: Nitric oxide and tumor necrosis factor-alpha production by Ixeris dentata in mouse peritoneal macrophages. J Ethnopharmacol. 82:217–222. 2002. View Article : Google Scholar : PubMed/NCBI
21	Kim SB, Kang OH, Keum JH, Mun SH, An HJ, Jung HJ, Hong SH, Jeong DM, Kweon KT and Kwon DY: Anti-inflammatory effect of Ixeris dentata on ultraviolet B-induced HaCaT keratinocytes. Nat Prod Sci. 18:60–66. 2012.(In Korean).
22	Kim MJ, Kim JS, Jeong DM, Han SS and Yu CY: Effect of antioxidant, antimutagenicity and anticancer of root extract from Ixeris dentata Nakai. Korean J Med Crop Sci. 10:222–229. 2002.
23	Kim JH, Lim HS, Ha H, Seo CS and Shin HK: Inulae flos and its compounds inhibit TNF-α- and IFN-γ-induced chemokine production in HaCaT human keratinocytes. Evid Based Complement Alternat Med. 2012:2803512012.PubMed/NCBI
24	Cha MR, Choi YH, Choi CW, Yoo DS, Kim YS, Choi SU, Kim YH and Ryu SY: New guaiane sesquiterpene lactones from Ixeris dentata. Planta Med. 77:380–382. 2011. View Article : Google Scholar
25	Choi JS, Young HS and Kim BW: Hypoglycemic and hypolipemic effects of Ixeris dentata in diabetic rats. Arch Pharm Res. 13:269–273. 1990. View Article : Google Scholar
26	Oh SH, Sung TH and Kim MR: Ixeris dentata extract maintains glutathione concentrations in mouse brain tissue under oxidative stress induced by kainic acid. J Med Food. 6:353–358. 2003. View Article : Google Scholar
27	Zidorn C: Sesquiterpene lactones and their precursors as chemosystematic markers in the tribe Cichorieae of the Asteraceae. Phytochemistry. 69:2270–2296. 2008. View Article : Google Scholar : PubMed/NCBI
28	Takashima A and Bergstresser PR: Impact of UVB radiation on the epidermal cytokine network. Photochem Photobiol. 63:397–400. 1996. View Article : Google Scholar : PubMed/NCBI
29	Grewe M, Trefzer U, Ballhorn A, Gyufko K, Henninger H and Krutmann J: Analysis of the mechanism of ultraviolet (UV) B radiation-induced prostaglandin E2 synthesis by human epidermoid carcinoma cells. J Invest Dermatol. 101:528–531. 1993. View Article : Google Scholar : PubMed/NCBI
30	An KP, Athar M, Tang X, Katiyar SK, Russo J, Beech J, Aszterbaum M, Kopelovich L, Epstein EH Jr, Mukhtar H and Bickers DR: Cyclooxygenase-2 expression in murine and human nonmelanoma skin cancers: implications for therapeutic approaches. Photochem Photobiol. 76:73–80. 2002. View Article : Google Scholar : PubMed/NCBI
31	Chen W, Tang Q, Gonzales MS and Bowden GT: Role of p38 MAP kinases and ERK in mediating ultraviolet-B induced cyclooxygenase-2 gene expression in human keratinocytes. Oncogene. 20:3921–3926. 2001. View Article : Google Scholar : PubMed/NCBI
32	Lin SK, Kok SH, Yeh FTC, Kuo MYP, Lin CC, Wang CC, Goldring SR and Hong CY: MEK/ERK and signal transducer and activator of transcription signaling pathways modulate oncostatin M-stimulated CCL2 expression in human osteoblasts through a common transcription factor. Arthritis Rheum. 50:785–793. 2004. View Article : Google Scholar
33	Mahns A, Wolber R, Stäb F, Klotz LO and Sies H: Contribution of UVB and UVA to UV-dependent stimulation of cyclooxygenase-2 expression in artificial epidermis. Photochem Photobiol Sci. 3:257–262. 2003. View Article : Google Scholar : PubMed/NCBI
34	Vayalil PK, Elmets CA and Katiyar SK: Treatment of green tea polyphenols in hydrophilic cream prevents UVB-induced oxidation of lipids and proteins, depletion of antioxidant enzymes and phosphorylation of MAPK proteins in SKH-1 hairless mouse skin. Carcinogenesis. 24:927–936. 2003. View Article : Google Scholar : PubMed/NCBI
35	Afaq F, Adhami VM and Ahmad N: Prevention of short-term ultraviolet B radiation-mediated damages by resveratrol in SKH-1 hairless mice. Toxicol Appl Pharmacol. 186:28–37. 2003.PubMed/NCBI
36	Cho JW, Lee KS and Kim CW: Curcumin attenuates the expression of IL-1β, IL-6, and TNF-α as well as cyclin E in TNF-α-treated HaCaT cells; NF-κB and MAPKs as potential upstream targets. Int J Mol Med. 19:469–474. 2007.