A novel PITX2c loss‑of‑function mutation underlies lone atrial fibrillation

  • Authors:
    • Yi‑Meng Zhou
    • Peng‑Xiang Zheng
    • Yi‑Qing Yang
    • Zhi‑Ming Ge
    • Wei‑Qiang Kang
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  • Published online on: August 2, 2013     https://doi.org/10.3892/ijmm.2013.1463
  • Pages: 827-834
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Abstract

Atrial fibrillation (AF) is the most common form of sustained cardiac arrhythmia responsible for substantial morbidity and significantly increased mortality rates. A growing body of evidence documents the important role of genetic defects in the pathogenesis of AF. However, AF is a heterogeneous disease and the genetic determinants for AF in an overwhelming majority of patients remain unknown. In the present study, a cohort of 100 unrelated patients with lone AF and a total of 200 unrelated, ethnically matched healthy individuals used as controls, were recruited. The whole coding exons and splice junctions of the pituitary homeobox 2c (PITX2c) gene, which encodes a paired‑like homeobox transcription factor required for normal cardiovascular morphogenesis, were sequenced in the 100 patients and 200 control subjects. The causative potential of the identified mutation of PITX2c was predicted by MutationTaster and PolyPhen‑2. The functional characteristics of the PITX2c mutation were assayed using a dual‑luciferase reporter assay system. Based on the results, a novel heterozygous PITX2c mutation (p.T97A) was identified in a patient with AF. The missense mutation was absent in the 400 reference chromosomes and was automatically predicted to be disease‑causing. Multiple alignments of PITX2c protein sequences across species revealed that the altered amino acid was completely conserved evolutionarily. Functional analysis demonstrated that the mutant PITX2c protein was associated with significantly decreased transcriptional activity when compared with its wild‑type counterpart. The findings of the present study firstly link the PITX2c loss‑of‑function mutation to lone AF, and provide novel insight into the molecular mechanisms underlying AF, suggesting the potential implications for the early prophylaxis and allele‑specific therapy of this common type of arrhythmia.
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October 2013
Volume 32 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhou YM, Zheng PX, Yang YQ, Ge ZM and Kang WQ: A novel PITX2c loss‑of‑function mutation underlies lone atrial fibrillation. Int J Mol Med 32: 827-834, 2013
APA
Zhou, Y., Zheng, P., Yang, Y., Ge, Z., & Kang, W. (2013). A novel PITX2c loss‑of‑function mutation underlies lone atrial fibrillation. International Journal of Molecular Medicine, 32, 827-834. https://doi.org/10.3892/ijmm.2013.1463
MLA
Zhou, Y., Zheng, P., Yang, Y., Ge, Z., Kang, W."A novel PITX2c loss‑of‑function mutation underlies lone atrial fibrillation". International Journal of Molecular Medicine 32.4 (2013): 827-834.
Chicago
Zhou, Y., Zheng, P., Yang, Y., Ge, Z., Kang, W."A novel PITX2c loss‑of‑function mutation underlies lone atrial fibrillation". International Journal of Molecular Medicine 32, no. 4 (2013): 827-834. https://doi.org/10.3892/ijmm.2013.1463