High interstitial fluid pressure promotes tumor cell proliferation and invasion in oral squamous cell carcinoma

Yu,Tao; Liu,Kun; Wu,Yingying; Fan,Jinchuan; Chen,Jianchao; Li,Chao; Zhu,Guiquan; Wang,Zhaohui; Li,Longjiang

doi:10.3892/ijmm.2013.1496

High interstitial fluid pressure promotes tumor cell proliferation and invasion in oral squamous cell carcinoma

Authors:
- Tao Yu
- Kun Liu
- Yingying Wu
- Jinchuan Fan
- Jianchao Chen
- Chao Li
- Guiquan Zhu
- Zhaohui Wang
- Longjiang Li
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Affiliations: Department of Head and Neck Oncology Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan 610041, P.R. China, State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Head and Neck Oncology Surgery, West China College of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: September 13, 2013 https://doi.org/10.3892/ijmm.2013.1496
Pages: 1093-1100

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Abstract

It has been shown that interstitial fluid pressure (IFP) is elevated in many solid tumors. The elevated IFP in tumors is responsible, at least in part, for the poor blood supply, inadequate delivery of therapeutic agents to solid tumors and poor treatment response in patients. The present study was carried out to examine alterations in malignant phenotypes in oral squamous cell carcinoma cells subjected to conditions mimicking IFP and to identify the relevant molecular mechanisms. We investigated tumor cell proliferation and invasion using SCC-4 and SCC-9 cells subjected to an increased extracellular pressure of 0, 15 and 30 mmHg in vitro. The results revealed that the increased IFP resulted in a marked increase in cancer cell proliferation, survival and invasion in vitro and altered the expression of >1,800 genes involved in invasion and metastasis, the heat shock pathway, the p38 and JNK signaling pathway, apoptosis and the cell growth and differentiation signaling pathway. These results suggest the important potential clinical application of measuring IFP, which can be used as a generic marker of prognosis and response to therapy.

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