Novel function of the poly(c)-binding protein α-CP2 as a transcriptional activator that binds to single-stranded DNA sequences

  • Authors:
    • Duk-Hee Kang
    • Kyu Young Song
    • Li-Na Wei
    • Ping-Yee Law
    • Horace H. Loh
    • Hack Sun Choi
  • View Affiliations

  • Published online on: September 11, 2013     https://doi.org/10.3892/ijmm.2013.1488
  • Pages: 1187-1194
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Abstract

α-complex protein 2 (α-CP2) is known as an RNA-binding protein that interacts in a sequence-specific manner with single-stranded polycytosine [poly(C)]. This protein is involved in various post-transcriptional regulations, such as mRNA stabilization and translational regulation. In this study, the full-length mouse α-CP2 gene was expressed in an insoluble form with an N-terminal histidine tag in Escherichia coli and purified for homogeneity using affinity column chromatography. Its identity was confirmed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Recombinant α-CP2 was expressed and refolded. The protein folding conditions for denatured α-CP2 were optimized. DNA and RNA electrophoretic mobility shift assays demonstrated that the recombinant α-CP2 is capable of binding to both single-stranded DNA and RNA poly(C) sequences. Furthermore, plasmids expressing α-CP2 activated the expression of a luciferase reporter when co-transfected with a single-stranded (pGL-SS) construct containing a poly(C) sequence. To our knowledge, this study demonstrates for the first time that α-CP2 functions as a transcriptional activator by binding to a single-stranded poly(C) sequence.
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November 2013
Volume 32 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kang D, Song KY, Wei L, Law P, Loh HH and Choi HS: Novel function of the poly(c)-binding protein α-CP2 as a transcriptional activator that binds to single-stranded DNA sequences. Int J Mol Med 32: 1187-1194, 2013
APA
Kang, D., Song, K.Y., Wei, L., Law, P., Loh, H.H., & Choi, H.S. (2013). Novel function of the poly(c)-binding protein α-CP2 as a transcriptional activator that binds to single-stranded DNA sequences. International Journal of Molecular Medicine, 32, 1187-1194. https://doi.org/10.3892/ijmm.2013.1488
MLA
Kang, D., Song, K. Y., Wei, L., Law, P., Loh, H. H., Choi, H. S."Novel function of the poly(c)-binding protein α-CP2 as a transcriptional activator that binds to single-stranded DNA sequences". International Journal of Molecular Medicine 32.5 (2013): 1187-1194.
Chicago
Kang, D., Song, K. Y., Wei, L., Law, P., Loh, H. H., Choi, H. S."Novel function of the poly(c)-binding protein α-CP2 as a transcriptional activator that binds to single-stranded DNA sequences". International Journal of Molecular Medicine 32, no. 5 (2013): 1187-1194. https://doi.org/10.3892/ijmm.2013.1488