H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells

Zhang,Qiang; Wang,Mei; Huang,Feng; Yang,Tingting; Cai,Jie; Zhang,Xu; Zhu,Wei; Qian,Hui; Xu,Wenrong

doi:10.3892/ijmm.2013.1532

H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells

Authors:
- Qiang Zhang
- Mei Wang
- Feng Huang
- Tingting Yang
- Jie Cai
- Xu Zhang
- Wei Zhu
- Hui Qian
- Wenrong Xu
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Affiliations: Key Institute of Clinical Laboratory Science, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China
Published online on: October 22, 2013 https://doi.org/10.3892/ijmm.2013.1532
Pages: 1465-1473

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Abstract

Mesenchymal stem cell (MSC) tropism to injured tissue sites in response to inflammation and wounds has been suggested. MSC activation and recruitment by Helicobacter pylori (H. pylori)-infected gastrointestinal epithelial cells has been demonstrated. As a component of the chronic gastritis microenvironment, MSCs play critical roles in the development of H. pylori-associated gastric mucosal lesions/malignancies. However, the mechanisms responsible for this process remain largely unknown. In this study, we demonstrate that H. pylori infection induces the differentiation of MSCs into cancer-associated fibroblast (CAF)-like cells. H. pylori-infected MSCs possessed an altered cytokine profile and induced epithelial-mesenchymal transition in gastric epithelial cells, leading to destroyed cell junctions, enhanced cell migration, reduced cell apoptosis and increased oncogenic potential. In conclusion, our findings indicate that H. pylori infection may cause gastric lesions/malignancies by inducing the differentiation of MSCs into CAFs and suggest a novel mechanism of action and role of MSCs in the development and progression of gastric cancer.

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