Effects of integrin ανβ3 on differentiation and collagen synthesis induced by connective tissue growth factor in human hypertrophic scar fibroblasts

  • Authors:
    • Xiaolong Hu
    • Na Li
    • Ke Tao
    • Xiaobing Fang
    • Jiaqi Liu
    • Yaojun Wang
    • Hongtao Wang
    • Jihong Shi
    • Yunchuan Wang
    • Peng Ji
    • Weixia Cai
    • Xiaozhi Bai
    • Xiongxiang Zhu
    • Juntao Han
    • Dahai Hu
  • View Affiliations

  • Published online on: August 22, 2014     https://doi.org/10.3892/ijmm.2014.1912
  • Pages: 1323-1334
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Abstract

CCN2 is a matricellular protein that appears to be important in scar formation. CCN2 mediates the pro-fibrotic effects in hypertrophic scars (HTSs) through an unknown mechanism. However, many activities of CCN2 protein are known to be mediated by direct binding to integrin receptors. In this study, we investigated the role of integrin ανβ3 in the differentiation of hypertrophic scar fibroblasts (HTSFs) induced by CCN2. The levels of integrin ανβ3 between normal skin and hypertrophic scar (HTS) tissues were compared, and integrin ανβ3 was found to be upregulated in HTS. CCN2 was shown to induce HTSF differentiation and collagen (COL) synthesis at the mRNA and protein levels. Based on these results, the expression of integrin ανβ3 was upregulated by CCN2 stimulation during HTSF differentiation. Blockade of integrin ανβ3 prevented CCN2-induced HTSF differentiation and COL synthesis. Furthermore, the CCN2-induced increase in contractility of the HTSF in COL lattices was inhibited by integrin ανβ3 blocking antibodies. HTSs were established in a rabbit ear model, and the inhibitor of integrin ανβ3 significantly improved the architecture of the rabbit ear scar. Results of the present study showed that integrin ανβ3 contributes to pro-fibrotic CCN2 signaling. Blocking this pathway may therefore be beneficial for the treatment of HTS.
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November-2014
Volume 34 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hu X, Li N, Tao K, Fang X, Liu J, Wang Y, Wang H, Shi J, Wang Y, Ji P, Ji P, et al: Effects of integrin ανβ3 on differentiation and collagen synthesis induced by connective tissue growth factor in human hypertrophic scar fibroblasts. Int J Mol Med 34: 1323-1334, 2014
APA
Hu, X., Li, N., Tao, K., Fang, X., Liu, J., Wang, Y. ... Hu, D. (2014). Effects of integrin ανβ3 on differentiation and collagen synthesis induced by connective tissue growth factor in human hypertrophic scar fibroblasts. International Journal of Molecular Medicine, 34, 1323-1334. https://doi.org/10.3892/ijmm.2014.1912
MLA
Hu, X., Li, N., Tao, K., Fang, X., Liu, J., Wang, Y., Wang, H., Shi, J., Wang, Y., Ji, P., Cai, W., Bai, X., Zhu, X., Han, J., Hu, D."Effects of integrin ανβ3 on differentiation and collagen synthesis induced by connective tissue growth factor in human hypertrophic scar fibroblasts". International Journal of Molecular Medicine 34.5 (2014): 1323-1334.
Chicago
Hu, X., Li, N., Tao, K., Fang, X., Liu, J., Wang, Y., Wang, H., Shi, J., Wang, Y., Ji, P., Cai, W., Bai, X., Zhu, X., Han, J., Hu, D."Effects of integrin ανβ3 on differentiation and collagen synthesis induced by connective tissue growth factor in human hypertrophic scar fibroblasts". International Journal of Molecular Medicine 34, no. 5 (2014): 1323-1334. https://doi.org/10.3892/ijmm.2014.1912