Open Access

S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer

  • Authors:
    • Jy-Ming Chiang
    • Reping Tan
    • Jen-Yi Wang
    • Jinn-Shium Chen
    • Yun-Shien Lee
    • Pao-Shiu Hsieh
    • Chung Rong Changchien
    • Jim-Ray Chen
  • View Affiliations

  • Published online on: January 12, 2015     https://doi.org/10.3892/ijmm.2015.2065
  • Pages: 675-683
  • Copyright: © Chiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Colorectal cancer (CRC) is a genetically heterogeneous disease with distinct morphological patterns. It has been shown that polypoid and ulcerative CRC displays different genetic alterations. In the present study, we aimed to investigate genes with differential expression patterns between ulcerative and polypoid CRC. cDNA microarray analysis was performed to compare the gene expression profiles in samples of ulcerative and polypoid CRC with paired normal mucosa samples. Potential candidate genes were further validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blot analysis and immunohistochemistry. The epigenetic regulation of gene expression was investigated using methylation-specific PCR (MSP). cDNA microarray analysis identified 11 upregulated and 14 downregulated genes which were differentially expressed in samples from both tumor types compared to the matched normal mucosa samples. Among these, S100P was the only upregulated gene preferentially associated with polypoid CRC (P=0.032). The samples of polypoid CRC displayed significantly higher S100P protein and mRNA expression levels than the samples of ulcerative CRC (P<0.05, respectively). Using semi-quantitative immunohistochemical analyses, S100P overexpression was found to be preferentially associated with polypoid CRC (24/30 vs. 14/40, P<0.001). The relative methylation level determined by MSP did not differ significantly between the samples of polypoid and ulcerative CRC (43.36 vs. 49.10%, P=0.168), indicating that promoter hypomethylation was not directly related to the upregulation of S100P mRNA. Our results demonstrate that the upregulation of S100P mRNA and protein expression is a predominant characteristic in polypoid CRC, whereas ulcerative CRC presents with a wide range of expression levels, indicating that S100P overexpression is not a key determinant in conferring invasion properties. The clinicopathological significance of S100P in CRC requires further investigation in well-controlled studies.
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March-2015
Volume 35 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chiang J, Tan R, Wang J, Chen J, Lee Y, Hsieh P, Changchien CR and Chen J: S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer. Int J Mol Med 35: 675-683, 2015.
APA
Chiang, J., Tan, R., Wang, J., Chen, J., Lee, Y., Hsieh, P. ... Chen, J. (2015). S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer. International Journal of Molecular Medicine, 35, 675-683. https://doi.org/10.3892/ijmm.2015.2065
MLA
Chiang, J., Tan, R., Wang, J., Chen, J., Lee, Y., Hsieh, P., Changchien, C. R., Chen, J."S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer". International Journal of Molecular Medicine 35.3 (2015): 675-683.
Chicago
Chiang, J., Tan, R., Wang, J., Chen, J., Lee, Y., Hsieh, P., Changchien, C. R., Chen, J."S100P, a calcium-binding protein, is preferentially associated with the growth of polypoid tumors in colorectal cancer". International Journal of Molecular Medicine 35, no. 3 (2015): 675-683. https://doi.org/10.3892/ijmm.2015.2065