Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats

  • Authors:
    • Taku Hebiguchi
    • Yoshihiro Mezaki
    • Mayako Morii
    • Ryo Watanabe
    • Kiwamu Yoshikawa
    • Mitsutaka Miura
    • Katsuyuki Imai
    • Haruki Senoo
    • Hiroaki Yoshino
  • View Affiliations

  • Published online on: January 12, 2015     https://doi.org/10.3892/ijmm.2015.2066
  • Pages: 724-730
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Abstract

Short bowel (SB) syndrome causes the malabsorption of various nutrients. Among these, vitamin A is important for a number of physiological activities. Vitamin A is absorbed by epithelial cells of the small intestine and is discharged into the lymphatic vessels as a component of chylomicrons and is delivered to the liver. In the present study, we used a rat model of SB syndrome in order to assess its effects on the expression of genes associated with the absorption, transport and metabolism of vitamin A. In the rats with SB, the intestinal mRNA expression levels of cellular retinol-binding protein II (CRBP II, gene symbol Rbp2) and apolipoprotein A-IV (gene symbol Apoa4) were higher than those in the sham-operated rats, as shown by RT-qPCR. Immunohistochemical analysis revealed that absorptive epithelial cells stained positive for both CRBP II and lecithin retinol acyltransferase, which are both required for the effective esterification of vitamin A. In the rats with SB, the retinol content in the ileum and the retinyl ester content in the jejunum were lower than those in the sham-operated rats, as shown by quantitative analysis of retinol and retinyl esters by high performance liquid chromatography. These results suggest that the elevated mRNA expression levels of Rbp2 and Apoa4 in the rats with SB contribute to the effective esterification and transport of vitamin A.
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March-2015
Volume 35 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hebiguchi T, Mezaki Y, Morii M, Watanabe R, Yoshikawa K, Miura M, Imai K, Senoo H and Yoshino H: Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats. Int J Mol Med 35: 724-730, 2015.
APA
Hebiguchi, T., Mezaki, Y., Morii, M., Watanabe, R., Yoshikawa, K., Miura, M. ... Yoshino, H. (2015). Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats. International Journal of Molecular Medicine, 35, 724-730. https://doi.org/10.3892/ijmm.2015.2066
MLA
Hebiguchi, T., Mezaki, Y., Morii, M., Watanabe, R., Yoshikawa, K., Miura, M., Imai, K., Senoo, H., Yoshino, H."Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats". International Journal of Molecular Medicine 35.3 (2015): 724-730.
Chicago
Hebiguchi, T., Mezaki, Y., Morii, M., Watanabe, R., Yoshikawa, K., Miura, M., Imai, K., Senoo, H., Yoshino, H."Massive bowel resection upregulates the intestinal mRNA expression levels of cellular retinol-binding protein II and apolipoprotein A-IV and alters the intestinal vitamin A status in rats". International Journal of Molecular Medicine 35, no. 3 (2015): 724-730. https://doi.org/10.3892/ijmm.2015.2066