CIP-36, a novel topoisomerase II-targeting agent, induces the apoptosis of multidrug-resistant cancer cells in vitro

  • Authors:
    • Bo Cao
    • Hong Chen
    • Ying Gao
    • Cong Niu
    • Yuan Zhang
    • Ling Li
  • View Affiliations

  • Published online on: January 14, 2015     https://doi.org/10.3892/ijmm.2015.2068
  • Pages: 771-776
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The need to overcome cancer multidrug resistance (MDR) has fueled considerable interest in the development of novel synthetic antitumor agents with cytotoxicity against cancer cell lines with MDR. In this study, we aimed to investigate CIP-36, a novel podophyllotoxin derivative, for its inhibitory effects on human cancer cells from multiple sources, particularly cells with MDR in vitro. The human leukemia cell line, K562, and the adriamycin-resistant subline, K562/A02, were exposed to CIP-36 or anticancer agents, and various morphological and biochemical properties were assessed by Hoechst 33342 staining under a fluorescence microscope. Subsequently, cytotoxicity, cell growth curves and the cell cycle were analyzed. Finally, the effects of CIP-36 on topoisomerase IIα (Topo IIα) activity were determined. Treatment with CIP-36 significantly inhibited the growth of the K562 and MDR K562/A02 cells. Our data demonstrated that CIP-36 induced apoptosis, inhibited cell cycle progression and inhibited Topo IIα activity. These findings suggest that CIP-36 has the potential to overcome the multidrug resistance of K562/A02 cells by mediating Topo IIα activity.
View Figures
View References

Related Articles

Journal Cover

March-2015
Volume 35 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Cao B, Chen H, Gao Y, Niu C, Zhang Y and Li L: CIP-36, a novel topoisomerase II-targeting agent, induces the apoptosis of multidrug-resistant cancer cells in vitro. Int J Mol Med 35: 771-776, 2015
APA
Cao, B., Chen, H., Gao, Y., Niu, C., Zhang, Y., & Li, L. (2015). CIP-36, a novel topoisomerase II-targeting agent, induces the apoptosis of multidrug-resistant cancer cells in vitro. International Journal of Molecular Medicine, 35, 771-776. https://doi.org/10.3892/ijmm.2015.2068
MLA
Cao, B., Chen, H., Gao, Y., Niu, C., Zhang, Y., Li, L."CIP-36, a novel topoisomerase II-targeting agent, induces the apoptosis of multidrug-resistant cancer cells in vitro". International Journal of Molecular Medicine 35.3 (2015): 771-776.
Chicago
Cao, B., Chen, H., Gao, Y., Niu, C., Zhang, Y., Li, L."CIP-36, a novel topoisomerase II-targeting agent, induces the apoptosis of multidrug-resistant cancer cells in vitro". International Journal of Molecular Medicine 35, no. 3 (2015): 771-776. https://doi.org/10.3892/ijmm.2015.2068